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Insulin, Serum/Plasma — Timed Post-Carbohydrate Challenge, Substance Concentration

Published: August 9, 2026Updated: August 16, 2026

Measures insulin in blood at a specified time after a carbohydrate challenge to evaluate how the pancreas responds to increased blood glucose.

Quick summary

["This timed insulin test helps evaluate pancreatic insulin release and the body's response to a carbohydrate load."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
25687-5
Short code
Insulin sp12 p chal SerPl-sCnc
Synonyms
Insulin sp12 p chal SerPl-sCnc
Category
hormone
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
2.6
Ref high
24.9
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
83525
SNOMED
413618004
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What is this test?

This test measures the amount of insulin in a blood sample. When it is collected as a timed post-carbohydrate test, the result shows how the pancreas responds after you drink a glucose-containing beverage.

Why might I need this test?

A timed insulin test may help evaluate insulin resistance, diabetes, or episodes of low blood glucose. It may be used alongside glucose measurements and other tests to assess how insulin and blood glucose change together.

How is the test performed?

A blood sample is collected at the exact interval specified by the laboratory. During a carbohydrate challenge test, you usually drink a measured glucose beverage, and blood is drawn at one or more scheduled times; your insulin result should be interpreted with the corresponding glucose results.

How do I prepare for the test?

Follow the laboratory's instructions about fasting, the carbohydrate challenge, and the exact collection time. Take medicines only as directed, and tell the healthcare team if you have recently used insulin or other glucose-lowering medicines.

Reference range

Fasting: 2.6-24.9 μIU/mLN/A

Specimen: serum

No special preparation usually required. Stay well hydrated. Inform your healthcare provider of all medications and supplements you are taking.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Insulin that remains elevated relative to glucose during the challenge may suggest reduced insulin sensitivity, which can occur with obesity, prediabetes, or type 2 diabetes. Insulin that is unexpectedly low or falls too far may contribute to low blood glucose. Results depend heavily on collection timing, symptoms, medicines, and the simultaneous glucose measurement.

Short name

Insulin

Frequently asked questions

Is this the same as a routine insulin test?
It may be. A timed post-carbohydte insulin test is collected at a specified point during a glucose challenge, while some laboratories also perform an insulin test without a challenge.
Why are insulin and glucose measured together?
The two results show how the body handles a carbohydrate load. Insulin alone is less informative because insulin levels and their interpretation depend on the glucose level at the same collection time.
Does the result diagnose diabetes?
No single insulin result usually does not diagnose diabetes. Your clinician will interpret it with glucose results, symptoms, medical history, other laboratory tests, and the timing of the sample.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy