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Insulin, Serum/Plasma — Substance Concentration

Published: August 9, 2026Updated: August 16, 2026

Measures the amount of insulin in a blood sample to help assess how the body regulates blood glucose.

Quick summary

["An insulin blood test is usually interpreted together with glucose levels to evaluate insulin production and glucose regulation."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
25691-7
Short code
Insulin sp1 p chal SerPl-sCnc
Synonyms
Insulin sp1 p chal SerPl-sCnc
Category
hormone
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
2.6
Ref high
24.9
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
83525
SNOMED
413618004
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What is this test?

This test measures insulin, a hormone made by the pancreas that helps cells take up glucose from the blood. It is performed using a serum or plasma blood sample.

Why might I need this test?

Your clinician may order this test when investigating high or low blood glucose, diabetes, insulin resistance, or symptoms related to abnormal insulin levels. It may also be measured during a glucose tolerance test or when evaluating how the body responds to a meal or glucose challenge.

How is the test performed?

A blood sample is drawn from a vein and placed in a collection tube. The laboratory measures the insulin concentration in the serum or plasma, often using an immunoassay.

How do I prepare for the test?

If your clinician requested a fasting test, follow their instructions, which may include avoiding food and drinks other than water for the specified period. If the test is part of a glucose tolerance test, follow the exact timing and preparation instructions provided.

Reference range

Fasting: 2.6-24.9 μIU/mLN/A

Specimen: serum

No special preparation usually required. Stay well hydrated. Inform your healthcare provider of all medications and supplements you are taking.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Insulin results must be interpreted with the accompanying glucose level, meal or glucose-challenge timing, medications, and clinical findings. Higher-than-expected insulin may occur with insulin resistance or hyperinsulinemia, while lower-than-expected insulin may indicate inadequate insulin production. Abnormal results do not by themselves diagnose a specific condition and should be reviewed by your clinician.

Short name

Insulin

Frequently asked questions

Is a fasting blood sample required for an insulin test?
It depends on why the test was ordered. A fasting sample may be requested, while another test may require timed measurements after eating or receiving glucose.
Why is my insulin result checked with glucose?
Insulin and glucose show different aspects of glucose regulation. Looking at both together is more useful than interpreting insulin alone.
Does a high insulin level always mean I have diabetes?
No. Elevated insulin can occur for several reasons, including insulin resistance, and the result must be considered alongside glucose and other clinical information.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy