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Insulin, Serum/Plasma — Substance Concentration

Published: August 9, 2026Updated: August 16, 2026

This blood test measures the amount of insulin in serum or plasma. It may be used to evaluate diabetes, insulin resistance, or symptoms related to abnormal insulin levels.

Quick summary

["Insulin testing helps assess how the body regulates blood sugar, usually alongside a glucose test."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
25694-1
Short code
Insulin sp4 p chal SerPl-sCnc
Synonyms
Insulin sp4 p chal SerPl-sCnc
Category
hormone
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
2.6
Ref high
24.9
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
83525
SNOMED
413618004
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What is this test?

This test measures insulin, a hormone made by the pancreas that helps glucose enter cells for energy. The result is usually interpreted together with blood glucose levels and your clinical situation.

Why might I need this test?

A clinician may order insulin testing to help evaluate diabetes or insulin resistance, particularly when fasting glucose or other blood sugar results are abnormal. It may also be used when symptoms suggest too much or too little insulin.

How is the test performed?

A blood sample is collected from a vein, and the laboratory measures the insulin concentration in the serum or plasma portion of the sample. The test may be performed as a standalone measurement or as part of a larger glucose evaluation.

How do I prepare for the test?

Follow your clinician's instructions about fasting and medication timing. Eating and recent physical activity can affect insulin levels, so do not fast or stop medicines unless the testing team specifically tells you to do so.

Reference range

Fasting: 2.6-24.9 μIU/mLN/A

Specimen: serum

No special preparation usually required. Stay well hydrated. Inform your healthcare provider of all medications and supplements you are taking.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Higher insulin levels can occur with insulin resistance, obesity, or diabetes, particularly when glucose is also elevated. Lower levels may occur with prolonged fasting, inadequate calorie intake, or excess insulin. Insulin results should be interpreted with glucose measurements, symptoms, medications, and the clinical context rather than alone.

Short name

Insulin

Frequently asked questions

Is fasting required for an insulin blood test?
It depends on why the test was ordered. Follow the specific instructions from your clinician, because fasting and meal timing can substantially affect the result.
Why is insulin measured with glucose?
Glucose shows the amount of sugar in the blood, while insulin shows how the body is responding to it. Together, the results provide more useful information than either test by itself.
Do medicines or eating affect the results?
Yes. Carbohydrate intake, recent meals, exercise, and insulin or other diabetes medicines can change insulin levels. Tell the testing team about relevant medicines and when you last ate.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy