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Insulin, Serum/Plasma — Post-Challenge, Mass Concentration

Published: August 9, 2026Updated: August 16, 2026

Measures the amount of insulin in a blood sample, often at a specified time after a glucose challenge, to help assess how the body regulates blood sugar.

Quick summary

["This insulin test helps evaluate blood-sugar regulation, and the collection time may be important when the result is part of a challenge test."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
12744-9
Short code
Insulin sp7 p chal SerPl-mCnc
Synonyms
Insulin sp7 p chal SerPl-mCnc
Category
hormone
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
2.6
Ref high
24.9
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
83525
SNOMED
413618004
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What is this test?

This test measures insulin, a hormone made by the pancreas that helps glucose move from the bloodstream into cells for energy. The sample may be collected at a specific time after a glucose or other challenge, so the timing listed with your test is important.

Why might I need this test?

Insulin testing may help evaluate unexplained high or low blood sugar, insulin resistance, or how the body responds to a glucose load. It is often interpreted together with glucose levels and, when appropriate, other tests.

How is the test performed?

A blood sample is drawn from a vein and tested in the laboratory to determine the concentration of insulin. If this is part of a challenge test, several samples may be collected at specified times.

How do I prepare for the test?

Follow the preparation and timing instructions provided by your healthcare professional, especially if the test is part of a glucose challenge. Fasting may be required for some insulin tests, while challenge testing may involve consuming a specified glucose product.

Reference range

Fasting: 2.6-24.9 μIU/mLN/A

Specimen: serum

No special preparation usually required. Stay well hydrated. Inform your healthcare provider of all medications and supplements you are taking.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Insulin results are interpreted with the matching glucose result and the timing of the sample. Insulin that is high when glucose is high may be associated with insulin resistance or impaired glucose regulation, while an insufficient insulin response may suggest reduced insulin production or action. Results must be assessed by a healthcare professional because conditions, medications, meals, and the timing of collection can affect them.

Short name

Insulin

Frequently asked questions

Does a high insulin level always mean I have diabetes?
No. Elevated insulin can occur with insulin resistance, obesity, certain medications, or other conditions, and it must be interpreted alongside glucose and the rest of your evaluation.
Why does the collection time matter?
Insulin changes after food or a glucose challenge. A sample collected at the wrong time may not be comparable with the intended result or the appropriate glucose value.
Will I need to fast before this test?
It depends on the test. Some insulin measurements require fasting, while timed challenge tests require following specific instructions and having samples taken at planned intervals.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy