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Insulin, Serum/Plasma — Substance Concentration

Published: August 9, 2026Updated: August 16, 2026

A blood test that measures insulin levels to help evaluate pancreatic function and investigate abnormalities in blood glucose.

Quick summary

["Insulin testing helps show how effectively the body regulates blood sugar."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
25698-2
Short code
Insulin sp8 p chal SerPl-sCnc
Synonyms
Insulin sp8 p chal SerPl-sCnc
Category
hormone
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
2.6
Ref high
24.9
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
83525
SNOMED
413618004
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What is this test?

This test measures the amount of insulin in a blood sample. Insulin is a hormone made by the pancreas that helps glucose enter cells for energy.

Why might I need this test?

Your clinician may order insulin testing to evaluate pancreatic function, investigate episodes of low blood sugar, or assess how your body responds to glucose. It may be used alongside glucose, C-peptide, and other diabetes tests.

How is the test performed?

A blood sample is drawn from a vein and analyzed by a laboratory method. If insulin is being measured as part of a glucose challenge or another timed test, samples may be collected before and after a specified stimulus.

How do I prepare for the test?

Follow your clinician's instructions about fasting and the timing of the test. Fasting insulin tests commonly require no food for about 8 to 12 hours, while water is usually allowed. Do not stop insulin or other medicines unless your clinician tells you to do so.

Reference range

Fasting: 2.6-24.9 μIU/mLN/A

Specimen: serum

No special preparation usually required. Stay well hydrated. Inform your healthcare provider of all medications and supplements you are taking.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

An insulin level that is higher or lower than expected may reflect differences in pancreatic function, food intake, stress, physical activity, or medication effects. When interpreted with glucose and other laboratory results, it can help clarify why blood sugar is too high or too low. A single insulin result is generally not enough to diagnose diabetes or another condition by itself.

Short name

Insulin

Frequently asked questions

Does this test diagnose diabetes?
Not by itself. Insulin is usually interpreted with fasting or standardized glucose measurements and the clinical findings.
Why might insulin be measured before and after a glucose drink?
Timed measurements show how strongly and for how long the pancreas releases insulin in response to glucose. This can help assess pancreatic function.
Do I need to stop taking insulin before the test?
No. Do not change insulin or other medication doses without instructions from your clinician, because doing so can affect both your health and the test results.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy