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Insulin, Serum/Plasma — 8-Hour Timed Concentration

Published: August 9, 2026Updated: August 16, 2026

Measures insulin in a serum or plasma sample collected at a specified time, usually as part of evaluating glucose metabolism.

Quick summary

["This timed insulin test is interpreted together with blood glucose, symptoms, medications, and the timing of the sample."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
50508-1
Short code
Insulin sp8 SerPl-aCnc
Synonyms
Insulin sp8 SerPl-aCnc
Category
hormone
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
2.6
Ref high
24.9
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
83525
SNOMED
413618004
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What is this test?

This test measures the amount of insulin in a blood sample. Insulin is a hormone made by the pancreas that helps glucose enter cells for energy. The test code indicates a timed collection; confirm with your care team whether the sample is meant to be taken 8 hours after eating or after a glucose load.

Why might I need this test?

A timed insulin test may be used to evaluate how the body responds to food or glucose. Your clinician may order it when investigating diabetes, insulin resistance, unusually high or low blood glucose, or symptoms related to low glucose.

How is the test performed?

A blood sample is drawn and separated into serum or plasma by the laboratory. Insulin is then measured using a laboratory assay. The result depends on the exact collection time and whether it follows a meal, fasting, or an oral glucose tolerance test.

How do I prepare for the test?

Follow the timing instructions provided by your clinician or laboratory, including any instructions about fasting or eating. The timing of insulin injections, diabetes medicines, and meals can affect the result, so tell the care team about them and do not stop prescribed medicines unless instructed.

Reference range

Fasting: 2.6-24.9 μIU/mLN/A

Specimen: serum

No special preparation usually required. Stay well hydrated. Inform your healthcare provider of all medications and supplements you are taking.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Insulin higher than expected for the collection conditions may occur with insulin resistance, obesity, metabolic syndrome, or after a glucose load. Insulin lower than expected, especially when glucose is high, may indicate that the pancreas is not producing enough insulin. A high insulin level together with low glucose may raise concern for excess insulin, including administered insulin, so results must be interpreted by a clinician using the timing and clinical context.

Short name

Timed insulin test

Frequently asked questions

Does one insulin result diagnose diabetes?
No. Insulin is usually interpreted with blood glucose results, symptoms, medical history, and medication use rather than by itself.
Why does the collection time matter?
Insulin changes naturally after eating and after glucose is given. An accurately timed sample helps the clinician interpret the result correctly.
Should I stop taking insulin before this test?
Do not change insulin or other diabetes medication unless your clinician specifically instructs you to do so. The testing team should confirm the preparation requirements.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy