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Insulin Level — 8-Hour Timed Measurement

Published: August 9, 2026Updated: August 17, 2026

A timed insulin blood test used to assess insulin levels and how the body responds to food, fasting, or administered insulin.

Quick summary

["The result is best interpreted with the collection timing, recent meals, and insulin doses because all can affect the insulin level."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 17, 2026Editorial policy

Key facts at a glance

LOINC
89908-8
Short code
Insulin taken in last 8H 8h
Synonyms
Insulin taken in last 8H 8h
Category
liver
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
10
Ref high
40
Critical range
— – 500 N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
84484
SNOMED
302774002
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What is this test?

This test measures the amount of insulin in a blood sample. Insulin is a hormone that helps glucose move into cells for energy and helps regulate blood glucose. The test code includes 8-hour timing, so the exact timing of the sample is important.

Why might I need this test?

Clinicians may order a timed insulin test when evaluating hypoglycemia, suspected insulin resistance, or how the body responds to a meal or glucose load. It may also be used in the care of diabetes, metabolic conditions, or symptoms related to excess insulin.

How is the test performed?

A blood sample is collected and sent to a laboratory, where insulin is measured with a laboratory assay. The result may be interpreted alone or alongside a simultaneous glucose measurement and other tests.

How do I prepare for the test?

Follow the timing instructions provided by your clinician, including whether you should fast and when the blood should be drawn. Do not skip meals, exercise excessively, or change insulin or other medicines unless your clinician instructs you to do so. Record the timing of your last meal and any insulin dose.

Reference range

Adult: 10-40 U/LN/A

Specimen: serum

No special preparation required. Avoid fatty meals before the test if possible.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

An insulin level that is unexpectedly high or low can reflect fasting, recent food intake, physical activity, or the use of insulin or other medicines. Patterns of elevated insulin may occur with insulin resistance, while unexpectedly low insulin can contribute to hypoglycemia. A single result cannot establish a diagnosis and should be interpreted with glucose levels, symptoms, medication use, and the collection timing.

Short name

Insulin (8-hour timing)

Frequently asked questions

Does the 8-hour timing mean I must fast for 8 hours?
Not necessarily. The code indicates 8-hour timing, but the required preparation may be fasting, a specific interval after eating, or a time after insulin administration. Confirm the exact protocol with the ordering clinician or laboratory.
Can I take my usual insulin before this test?
Do not change your insulin routine unless your clinician gives specific instructions. If a dose is permitted, report its type, amount, and exact time because injected insulin can affect the result.
What other information helps interpret the result?
The collection time, last meal and meal size, recent exercise, symptoms, insulin doses, medicines, and any glucose measured at the same time can help the clinician interpret the insulin level.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 17, 2026. · Read our editorial policy