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Intrinsic Factor Antibody Test, Blood

Published: August 9, 2026Updated: August 16, 2026

Detects antibodies against intrinsic factor, a stomach protein needed for vitamin B12 absorption, to help evaluate causes of vitamin B12 deficiency.

Quick summary

["A positive result may support pernicious anemia or autoimmune gastritis as the cause of vitamin B12 deficiency."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
52762-2
Short code
Intrinsic TEG Pnl Bld
Synonyms
Intrinsic TEG Pnl Bld
Category
liver
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What is this test?

This blood test looks for antibodies directed against intrinsic factor. Intrinsic factor is made by the stomach and helps the body absorb vitamin B12.

Why might I need this test?

Your clinician may order this test when vitamin B12 deficiency is suspected, particularly if you have anemia, numbness or tingling, balance problems, or other symptoms that could be related to a lack of vitamin B12.

How is the test performed?

A blood sample is collected and tested for antibodies that interfere with intrinsic factor. The laboratory may report the result as positive or negative, with additional information from the full test report if needed.

How do I prepare for the test?

This test usually requires only a routine blood draw and does not generally require fasting. Follow any preparation instructions provided by your healthcare professional.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Intrinsic factor antibodies may be present in pernicious anemia, an autoimmune condition in which the stomach cannot make enough intrinsic factor to absorb vitamin B12 normally. They may also occur in autoimmune gastritis without current symptoms. Results should be interpreted together with vitamin B12 levels, other laboratory findings, and your symptoms.

Short name

Intrinsic factor antibody test

Frequently asked questions

What does a positive intrinsic factor antibody result mean?
It may indicate pernicious anemia or autoimmune gastritis, both of which can interfere with vitamin B12 absorption. Your clinician will interpret the result with your other test findings.
Can I have a positive result without pernicious anemia?
Yes. Antibodies may occasionally be found in people who do not have pernicious anemia, so a positive result does not establish that diagnosis by itself.
Does a negative result rule out pernicious anemia?
No. A negative result makes pernicious anemia less likely, but rare antibody-negative cases can occur. Your clinician may consider vitamin B12 and other tests when deciding on a diagnosis.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy