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Serum Iron with Proportionate Non-Saturated Binding Capacity (Iron-NSBC Ratio)

Published: August 9, 2026Updated: August 17, 2026

This test measures your body’s iron levels and how much of your iron is available to bind with proteins, helping assess iron status and potential deficiencies or overloads.

Quick summary

["This test helps diagnose iron-related blood disorders by evaluating iron availability and binding capacity in your serum."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 17, 2026Editorial policy

Key facts at a glance

LOINC
75689-0
Short code
Iron Pnl SerPl
Synonyms
Iron Pnl SerPl
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
60
Ref high
170
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
83540
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Matters

Iron is an essential mineral for making red blood cells and maintaining energy levels. If your body doesn’t have enough iron, you may develop anemia or fatigue. This test helps determine if your iron levels are too low or too high, which can affect your overall health.

What to Expect Before the Test

No special preparation is needed for this test. You’ll have a small needle inserted into your arm to draw blood. It usually takes a few minutes, and you may feel a slight pinch. If you’re on blood thinners or have a history of bleeding, let your healthcare provider know before the test.

What the Results Mean

The results combine your serum iron levels with a measure of how much iron is bound to proteins in your blood. Low results may indicate iron deficiency, while high results could suggest iron overload or other conditions. Your doctor will interpret these results in the context of your overall health.

Common Conditions This Test Helps Diagnose

This test is often used to diagnose iron deficiency anemia, hemochromatosis (iron overload), or other conditions affecting iron metabolism. It can also help monitor treatment for these conditions.

Reference range

NCBI: Serum Iron Male 65-180; Female 50-170 µg/dLN/A

Specimen: serum

Fasting for 12 hours preferred. Iron supplements should be stopped 24 hours before if possible.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

Iron and Binding Capacity Test

Frequently asked questions

How often do I need this test?
Your doctor may recommend this test if you have symptoms like fatigue, pale skin, weakness, or unexplained shortness of breath. It may also be part of routine blood work for certain health conditions or during pregnancy. Follow your healthcare provider’s advice on how often to get tested.
What are the typical reference ranges for this test?
Reference ranges can vary by lab and individual health. Your results will be compared to your specific lab’s standards and clinical context, not a single set of numbers. Your doctor will discuss what your results mean for you.
Can I eat or drink anything before this test?
You can eat and drink normally before this test, as no special diet or fasting is required. However, always follow your healthcare provider’s instructions for any medications or supplements you’re taking.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 17, 2026. · Read our editorial policy