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Iron (Serum) with Bromocresol Purple Colorimetric Method

Published: August 9, 2026Updated: August 17, 2026

This test measures the amount of iron present in your blood serum, which is important for making hemoglobin—the protein in red blood cells that carries oxygen throughout your body.

Quick summary

["Iron levels help assess your risk of anemia or iron overload."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 17, 2026Editorial policy

Key facts at a glance

LOINC
50190-8
Short code
Iron+TIBC Pnl SerPl
Synonyms
Iron+TIBC Pnl SerPl
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
60
Ref high
170
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
83540
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why Is This Test Done?

Your healthcare provider may order this test to check for iron deficiency (low iron levels) or iron overload (high iron levels), which can cause fatigue, weakness, or other symptoms. It may also be used in monitoring conditions like hemochromatosis or during pregnancy to ensure proper iron levels for fetal development.

What Does the Test Involve?

A blood sample is drawn from a vein in your arm. The sample is then processed to measure iron concentration using a colorimetric method with bromocresol purple, which changes color based on the iron amount. Results are compared to general reference ranges for healthy adults.

What Are Common Reasons for Testing?

Common reasons include diagnosing or monitoring iron deficiency anemia, evaluating unexplained fatigue, checking for hemochromatosis (a genetic condition causing iron overload), or assessing nutritional status.

What Might Affect Results?

Several factors can influence iron levels, such as diet (high intake of iron-rich foods or supplements), blood loss (e.g., from heavy periods or injuries), certain medications (e.g., antacids, blood thinners), or underlying health conditions like celiac disease or inflammatory bowel disease.

Reference range

NCBI: Serum Iron Male 65-180; Female 50-170 µg/dLN/A

Specimen: serum

Fasting for 12 hours preferred. Iron supplements should be stopped 24 hours before if possible.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Serum Iron

Frequently asked questions

What Are Normal Results?
Normal iron levels can vary slightly by lab, but generally, serum iron tests help identify whether iron stores are adequate, depleted, or excessive. Low levels may indicate anemia or poor absorption, while high levels could signal hemochromatosis or other metabolic disorders.
How Do I Prepare for This Test?
No special preparation is usually needed, but avoid taking iron supplements or certain medications (like antacids) 24 hours before the test if instructed by your provider.
What If I Have Questions About My Results?
If your results are outside the expected range, discuss them with your healthcare provider. They may recommend further testing, dietary adjustments, or treatment options based on your symptoms and medical history.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 17, 2026. · Read our editorial policy