Iron (Serum) with Bromocresol Purple Colorimetric Method
This test measures the amount of iron present in your blood serum, which is important for making hemoglobin—the protein in red blood cells that carries oxygen throughout your body.
["Iron levels help assess your risk of anemia or iron overload."]
Key facts at a glance
- LOINC
- 50190-8
- Short code
- Iron+TIBC Pnl SerPl
- Synonyms
- Iron+TIBC Pnl SerPl
- Category
- chemistry
- Sample
- serum
- Container
- Red-top tube (SST)
- Units
- N/A
- Ref low
- 60
- Ref high
- 170
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- CPT
- 83540
- Specimen volume
- 5 mL
- Stability
- Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
- Hemolysis max
- 500
- Lipemia max
- 1000
- Icterus max
- 500
- TAT
- 4–24 hours
- Order
- routine
Why Is This Test Done?
Your healthcare provider may order this test to check for iron deficiency (low iron levels) or iron overload (high iron levels), which can cause fatigue, weakness, or other symptoms. It may also be used in monitoring conditions like hemochromatosis or during pregnancy to ensure proper iron levels for fetal development.
What Does the Test Involve?
A blood sample is drawn from a vein in your arm. The sample is then processed to measure iron concentration using a colorimetric method with bromocresol purple, which changes color based on the iron amount. Results are compared to general reference ranges for healthy adults.
What Are Common Reasons for Testing?
Common reasons include diagnosing or monitoring iron deficiency anemia, evaluating unexplained fatigue, checking for hemochromatosis (a genetic condition causing iron overload), or assessing nutritional status.
What Might Affect Results?
Several factors can influence iron levels, such as diet (high intake of iron-rich foods or supplements), blood loss (e.g., from heavy periods or injuries), certain medications (e.g., antacids, blood thinners), or underlying health conditions like celiac disease or inflammatory bowel disease.
Reference range
NCBI: Serum Iron Male 65-180; Female 50-170 µg/dLN/A
Fasting for 12 hours preferred. Iron supplements should be stopped 24 hours before if possible.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
◆Associated findings1
Short name
Serum Iron
Frequently asked questions
What Are Normal Results?
How Do I Prepare for This Test?
What If I Have Questions About My Results?
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 17, 2026. · Read our editorial policy