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Iron Total Serum (Iron Concentration in Serum)

Published: August 9, 2026Updated: August 17, 2026

This test measures the amount of iron present in your blood serum, which helps assess iron stores and identify potential iron deficiency or overload conditions.

Quick summary

["Iron levels can indicate whether you have enough iron to make healthy red blood cells or if you may need treatment."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 17, 2026Editorial policy

Key facts at a glance

LOINC
2505-6
Short code
Iron/TIBC SerPl
Synonyms
Iron/TIBC SerPl
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
60
Ref high
170
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
83540
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Might Be Ordered

Your healthcare provider may recommend this test if you have symptoms like fatigue, pale skin, weakness, or shortness of breath, which can be signs of iron deficiency. It may also be used to monitor iron levels in people with chronic conditions like anemia, certain gastrointestinal disorders, or those receiving blood transfusions. Additionally, it could be part of a routine check-up for individuals at higher risk for iron-related issues.

What to Expect Before the Test

No special preparation is usually needed for this test. You may be asked to fast for 8–12 hours before the draw if your lab requires it, but this is not always the case. Bring any medications or supplements you’re taking, as they can sometimes affect results.

What the Test Involves

A healthcare professional will draw blood from a vein in your arm using a small needle. You may feel a slight pinch, but the process is quick and typically takes a few minutes. The blood sample is then sent to a lab for analysis.

What the Results Mean

Results help determine whether your iron stores are adequate or if you have low (iron deficiency) or high (iron overload) levels. Your doctor will compare your results to standard reference ranges and consider your symptoms, medical history, and other test results to make a diagnosis.

Reference range

NCBI: Serum Iron Male 65-180; Female 50-170 µg/dLN/A

Specimen: serum

Fasting for 12 hours preferred. Iron supplements should be stopped 24 hours before if possible.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Iron

Frequently asked questions

How long does it take to get my iron test results?
Results are typically available within 1–3 business days, depending on your lab’s processing time. Some labs may provide preliminary results sooner, but your final report will include detailed interpretations from your healthcare provider.
Can I take iron supplements before my test?
If your provider hasn’t instructed otherwise, avoid taking iron supplements or certain medications (like multivitamins with iron) for at least 24 hours before the test, as they can affect the accuracy of your results. Always follow your lab’s specific guidelines.
What are common symptoms of iron deficiency?
Common symptoms include persistent fatigue, pale or yellowish skin, brittle nails, hair loss, dizziness, or an unusual craving for non-food items (pica). If you experience these symptoms, especially combined with other signs like shortness of breath or rapid heartbeat, talk to your doctor about testing.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 17, 2026. · Read our editorial policy