Skip to content

Serum Iron and Transferrin Saturation (Iron Binding Capacity)

Published: August 9, 2026Updated: August 17, 2026

This test measures two key parts of your body’s iron balance: how much iron is in your blood and how well your protein called transferrin can carry iron.

Quick summary

["Helps assess iron deficiency or overload, which can affect energy and health."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 17, 2026Editorial policy

Key facts at a glance

LOINC
13452-8
Short code
Iron/Transferrin SerPl
Synonyms
Iron/Transferrin SerPl
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
60
Ref high
170
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
83540
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why It’s Done

Your doctor may order these tests if you have symptoms like fatigue, pale skin, unusual tiredness, or if they suspect conditions like anemia, hemochromatosis (iron overload), or other blood disorders. They might also check iron levels if you’re pregnant, have frequent infections, or have an unusual appetite for non-food items (pica).

What to Expect

A small blood sample is taken from a vein in your arm. The blood is sent to a lab where it’s analyzed to measure iron levels and how much of your transferrin protein is saturated with iron. Results help determine if you need iron supplements or other treatments.

Normal vs. Abnormal Results

There are no standard reference ranges provided here since they can vary by lab. Your results will be interpreted by your healthcare provider based on your overall health and symptoms. Low results may indicate iron deficiency, while high results could suggest iron overload or other conditions.

Common Conditions Linked to These Tests

Low iron levels (low iron or high transferrin saturation) often point to anemia or iron deficiency, which can cause fatigue, weakness, or pale skin. High iron levels (low transferrin saturation) may indicate hemochromatosis or other iron storage disorders. These tests also help monitor conditions like chronic kidney disease or certain infections.

Reference range

NCBI: Serum Iron Male 65-180; Female 50-170 µg/dLN/A

Specimen: serum

Fasting for 12 hours preferred. Iron supplements should be stopped 24 hours before if possible.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Iron Studies (Serum)

Frequently asked questions

What questions might you have about this test?
Will I need to prepare for this test? Most blood tests don’t require special preparation, but you may be asked to fast for a few hours before your blood draw if iron levels are being checked in a different way (like a fasting test).
How long will it take to get my results?
Results are typically available within a few days to a week, depending on your lab. Your doctor will discuss them with you during your follow-up visit.
Can I take iron supplements without checking my levels first?
No. Taking iron supplements without medical guidance can be harmful, especially if you have iron overload. Always consult your doctor before starting any supplements, including iron.
  1. 1
  2. 2
  3. 3

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 17, 2026. · Read our editorial policy