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Urine Ketones (Qualitative, 24-Hour Collection)

Published: August 9, 2026Updated: August 16, 2026

This test checks for the presence of ketones in your urine over a 24-hour period, which can indicate your body is breaking down fat for energy instead of glucose.

Quick summary

["Ketones in urine may signal metabolic stress, such as uncontrolled diabetes or fasting."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
27065-2
Short code
Ketones 2h p chal Ur Ql
Synonyms
Ketones 2h p chal Ur Ql
Category
chemistry
Sample
urine
Container
Sterile urine container
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
10 mL
Stability
Room temperature: 2h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

This test is done to give your doctor important information about your health. It can help find a health problem early, show how well part of your body is working, or check how well a treatment is going. Knowing these details helps your care team choose the safest and most effective plan for you.

Your healthcare provider may order this test to monitor conditions like diabetes, starvation, or metabolic disorders where fat breakdown (ketosis) occurs. It helps assess whether your body is efficiently using energy sources or if additional support is needed.

Before the test, your care team will give you clear instructions on how to prepare. You may be asked to avoid eating or drinking for a certain amount of time, or to pause some medicines. Wear comfortable, loose clothing, bring a list of your current medications, and arrive a little early so you have time to check in and ask any questions.

You’ll collect all urine produced in a 24-hour period in a sterile container. Drink plenty of fluids to ensure adequate urine output. Avoid certain medications or supplements that might interfere with results.

During the test, a trained member of your care team will walk you through each step and answer your questions along the way. The test is designed to be safe, and most people tolerate it well. It usually takes only a short time, and you can normally go home the same day unless your doctor tells you otherwise.

The collected urine sample is tested for the presence of ketones using a chemical reaction that detects their concentration. Results are reported as either positive (ketones present) or negative (no ketones detected).

Your results will help your doctor better understand your health. Results within the normal range may mean everything looks as expected, while results outside that range can point to a condition that needs a closer look. Your doctor will contact you to explain what your results mean and discuss any next steps in your care.

Positive results suggest elevated ketone levels, which may indicate conditions like diabetic ketoacidosis, starvation, or prolonged fasting. Negative results mean no significant ketones were detected in your urine.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance: Ketones in Urine Can Signal Underlying Health Concerns

Short name

Urine Ketones (24h)

Frequently asked questions

Ketones in urine are typically a sign of metabolic stress. In diabetes, high blood sugar can lead to fat breakdown, producing ketones that may cause dangerous acidity in the blood (ketoacidosis). In non-diabetic cases, prolonged fasting or excessive exercise can also trigger ketones.
Why might my doctor recommend this test for me, and how could the results help improve my care?
Your doctor may recommend this test if you have symptoms like excessive thirst, frequent urination, nausea, or unexplained weight loss, especially if you have diabetes or a history of metabolic issues.
A positive result usually means the test found the sign or condition it was looking for. It does not always mean you are seriously ill. Your doctor will review the result together with your symptoms, medical history, and any other tests, and may order follow-up testing to confirm the finding and decide on the best next steps for your treatment.
A positive result means ketones were found in your urine. This could indicate your body is breaking down fat for energy, which may require medical attention depending on your health status and symptoms.
No specific diet is required before the test, but avoid high-fat meals or supplements that might affect ketone levels. Drink water to help produce urine, but avoid alcohol or caffeine, as they can influence results.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy