Serum or plasma protein electrophoresis with bromocresol green dye binding

What This Test Measures

Electrophoresis separates blood proteins into different fractions (e.g., albumin, globulins) based on size and charge. Bromocresol green binds to albumin, allowing for a visual or quantitative assessment of its concentration relative to other proteins. This helps detect abnormalities in protein distribution that may signal underlying conditions.

Why It’s Done

Doctors may order this test if you have symptoms like swelling, unexplained weight loss, fatigue, or if they suspect conditions such as nephrotic syndrome, multiple myeloma, or chronic liver disease. It’s also used in screening for certain genetic disorders or monitoring treatment responses.

Sample Collection and Preparation

A blood sample is drawn from a vein, usually in the arm. The serum or plasma is then processed and analyzed using electrophoresis with the bromocresol green dye. Results are often expressed as a ratio or percentage of protein fractions.

Common Conditions Evaluated

This test can help identify conditions affecting protein levels, such as kidney disease (e.g., nephrotic syndrome), liver disease, chronic infections, or certain cancers (e.g., multiple myeloma). It may also be used in research or specialized diagnostics for rare genetic disorders.