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Serum or Plasma Substance Concentration (Bromocresol Green Dye Binding)

Published: August 9, 2026Updated: August 16, 2026

This test measures how much of a specific protein in your blood binds to bromocresol green dye, which helps assess protein levels or liver function.

Quick summary

["This test may help diagnose or monitor conditions affecting protein synthesis or liver health."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
7449-2
Short code
Lamb BasoBnd Ab Qn
Synonyms
Lamb BasoBnd Ab Qn
Category
chemistry
Sample
whole-blood
Container
Lavender-top (EDTA)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
lhr
Specimen volume
3 mL
Stability
Room temperature: 4h, Refrigerated: 24h
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

What This Test Measures

The bromocresol green (BCG) dye binding test evaluates the concentration of a protein in your blood that binds to this dye. The dye’s ability to bind is influenced by the protein’s presence, often used to indirectly assess albumin or other protein-related functions.

Why It’s Done

Your healthcare provider may order this test if you have symptoms of liver disease, kidney problems, malnutrition, or other conditions affecting protein levels. It can also help monitor chronic conditions like cirrhosis or nephrotic syndrome.

Sample Collection and Preparation

A blood sample is drawn from a vein, typically in your arm. The sample is processed to separate serum or plasma, where the dye-binding reaction occurs. Results are compared to a standard curve to determine the concentration.

Possible Results and Interpretation

While exact reference ranges vary by lab, lower than expected binding may indicate protein loss (e.g., due to kidney disease) or liver dysfunction. Higher than expected binding could suggest dehydration or other conditions affecting protein volume.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance: This test is less commonly used today but historically helped assess albumin levels or detect liver dysfunction by observing dye binding patterns. It may still be employed in specialized settings or research contexts to evaluate protein-related abnormalities.

Short name

BCG Dye Binding Test

Frequently asked questions

What does a low or high BCG dye binding result mean?
A low result might suggest conditions like severe liver disease, kidney failure, or malnutrition where proteins are lost or not produced properly. A high result could indicate dehydration or other factors affecting plasma volume.
Is this test painful?
The blood draw itself may cause mild discomfort, but the test itself is non-invasive. Most people report minimal pain or irritation at the injection site.
How long does it take to get results?
Results are typically available within 1–3 business days, depending on lab processing times and your healthcare provider’s workflow.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy