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Total Protein with Albumin and Globulin Profile (Serum/Plasma)

Published: August 9, 2026Updated: August 16, 2026

This test measures your blood’s total protein and separates it into albumin and globulin components, which help assess protein balance and potential liver, kidney, or immune system issues.

Quick summary

["Helps identify protein disorders or organ dysfunction by evaluating protein distribution in your blood."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
57181-0
Short code
LA Nt dPL/LA Nt HPL PPP-Rto
Synonyms
LA Nt dPL/LA Nt HPL PPP-Rto
Category
chemistry
Sample
plasma
Container
Lavender-top (EDTA) or Green-top (Heparin)
Units
N/A
Fasting
true
Population
adult
Sex
all
Age range
18–65 years
Method
coag
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Matters

Protein is vital for many bodily functions, including transporting nutrients, maintaining fluid balance, and supporting immune responses. Albumin, the most abundant protein in blood, helps keep fluids in circulation, while globulins play roles in immunity and blood clotting. Abnormal levels can signal conditions like liver disease, kidney problems, or infections.

How the Test Works

Your blood sample is analyzed to measure total protein concentration and then divided into albumin and globulin fractions. Methods may include automated chemistry or electrophoresis, depending on the lab’s standard procedure.

Common Conditions Monitored

This test is often used to diagnose or monitor conditions like cirrhosis (liver disease), nephrotic syndrome (kidney disease), chronic infections, or autoimmune disorders. Low albumin may indicate malnutrition or severe illness, while elevated globulins could suggest inflammation or certain cancers.

What to Expect Before the Test

No special preparation is needed, but you may be asked to fast for 8–12 hours if your doctor specifies a fasting blood test for other components. Wear comfortable clothing and arrive with your doctor’s instructions if fasting is required.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Total Protein and Fraction Breakdown

Frequently asked questions

What does a low total protein level mean?
Low total protein or albumin may indicate liver disease, kidney problems, malnutrition, or severe infections. Globulin levels can also be low if your immune system isn’t functioning well. Always discuss results with your healthcare provider, as they’ll consider your full medical history and other test results.
What does a high globulin level suggest?
Elevated globulins often point to chronic inflammation, autoimmune diseases, or certain cancers. Your doctor may recommend further testing, such as blood counts or imaging, to explore underlying causes.
Can diet affect these test results?
Yes, diet can influence protein levels. High-protein diets may temporarily raise total protein, while low-protein diets could lower it. If fasting is required, avoid high-protein foods before your blood draw to ensure accurate results.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy