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Serum Vitamin D (25-hydroxyvitamin D) concentration

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of vitamin D in your blood, which is important for bone health, immune function, and overall well-being. Low levels may indicate deficiency, which can affect how your body absorbs calcium and supports muscle and nerve function.

Quick summary

["Vitamin D helps your body use calcium and phosphorus to build strong bones and supports immune health."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
110974-3
Short code
LASV IgM Msmt Ser
Synonyms
LASV IgM Msmt Ser
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
82784
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why Is This Test Done?

Your healthcare provider may order this test if you have symptoms of vitamin D deficiency, such as muscle weakness, bone pain, or frequent illnesses. It may also be recommended for people at higher risk, including those with limited sun exposure, certain medical conditions, or those taking medications that interfere with vitamin D absorption.

What to Expect Before the Test

No special preparation is usually needed, but you may be asked to fast for 8–12 hours before the test if your lab uses a specific protocol. Wear comfortable clothing and plan to stay near the testing site for about 15 minutes while blood is drawn.

What Happens During the Test?

A healthcare professional will insert a small needle into a vein in your arm to draw blood. You may feel a slight pinch, but it usually doesn’t cause significant discomfort. The blood sample is then sent to a lab for analysis.

What Are the Results?

Results are typically reported as a numerical value representing the concentration of vitamin D in your blood. Your provider will interpret the results in relation to your health status and may recommend adjustments to diet, supplements, or lifestyle if needed.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Vitamin D plays a critical role in preventing bone diseases like osteoporosis, supporting immune function, and potentially reducing the risk of certain chronic conditions. Low levels may lead to weakened bones, increased infection risk, or other health issues. High levels can sometimes cause symptoms like nausea, fatigue, or kidney problems, so monitoring is important.

Short name

Vitamin D Level (Serum)

Frequently asked questions

How long does it take to get my vitamin D test results?
Results are usually available within 1–3 business days, depending on your lab’s processing time. Your provider will contact you once they are ready, so check with them for an estimated timeline.
Can I eat or drink anything before my vitamin D blood test?
Most labs do not require fasting for this test, but follow any specific instructions your provider gives you. If fasting is needed, avoid food and liquids (except water) for 8–12 hours before the test to ensure accurate results.
What does a low vitamin D level mean?
A low vitamin D level suggests you may not be getting enough from sunlight, diet, or supplements. Your provider may recommend increasing sun exposure, eating vitamin D-rich foods (like fatty fish or fortified dairy), or taking supplements under medical guidance to raise your levels safely.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy