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Lymphocyte Subset Analysis by Flow Cytometry (Manual Count)

Published: August 9, 2026Updated: August 16, 2026

This test measures the number of different types of white blood cells called lymphocytes in your blood, which helps assess immune function and identify potential immune disorders.

Quick summary

["This test helps doctors understand your immune system’s balance and spot problems like infections, immune deficiencies, or blood disorders."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
11275-5
Short code
Lg Gran Lymphs NFr Bld Manual
Synonyms
Lg Gran Lymphs NFr Bld Manual
Category
chemistry
Sample
whole-blood
Container
Lavender-top (EDTA)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
manual-count
Specimen volume
3 mL
Stability
Room temperature: 4h, Refrigerated: 24h
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

What Does This Test Measure?

Your blood sample is analyzed to count specific lymphocyte subsets, including T-cells, B-cells, and natural killer (NK) cells. This manual method provides detailed counts of these immune cells, which can be used to evaluate immune health and diagnose conditions like lymphoma or autoimmune diseases.

Why Might You Need This Test?

Doctors may order this test if you have symptoms like frequent infections, unexplained fatigue, or if you’re undergoing treatment for a blood-related condition. It helps track immune responses before or after treatments like chemotherapy or immunotherapy.

How Is the Test Performed?

A small blood sample is drawn from your arm. The sample is then processed manually to count and classify different lymphocyte types, ensuring precise measurements of immune cell populations.

What Are the Possible Results?

Results show the absolute counts of each lymphocyte subset, helping identify imbalances or abnormalities in immune cell numbers that may require further medical evaluation.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

This test is clinically significant for diagnosing and monitoring conditions affecting the immune system, such as leukemia, lymphoma, or immune deficiencies. It also aids in assessing the effectiveness of treatments that target immune cells.

Short name

Manual Lymphocyte Count

Frequently asked questions

How long will I need to wait for my results?
Results from manual lymphocyte counts may take longer to process compared to automated tests, often requiring 2–5 business days depending on lab workload. Follow-up with your healthcare provider for next steps.
What should I do if my results are abnormal?
If your lymphocyte counts are outside expected ranges, your doctor may recommend additional tests or treatments to address the underlying cause, such as infections, immune disorders, or other health conditions.
Can I eat or drink anything before this test?
No special preparation is needed for this test. However, inform your doctor about any medications or supplements you’re taking, as they may affect blood cell counts.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy