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Total Protein with Albumin/GLOBulin Separation (Serum)

Published: August 9, 2026Updated: August 16, 2026

This test measures your blood’s total protein levels and breaks down the protein fractions—albumin and globulins—into separate components for detailed analysis.

Quick summary

["Understanding your protein balance helps identify issues like kidney disease, liver problems, or nutritional deficiencies."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
94287-0
Short code
LGI1 IgG Ser Ql CBA IFA
Synonyms
LGI1 IgG Ser Ql CBA IFA
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
cba-ifa
CPT
82784
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What Does This Test Measure?

Total protein combines all proteins in your blood, including albumin (the main carrier protein) and globulins (antibodies and other proteins). This test often uses electrophoresis to separate these fractions for closer inspection.

Why Might You Need This Test?

Your doctor may order this test to check for conditions affecting protein production, breakdown, or balance, such as kidney disease, liver disorders, or chronic inflammation.

How Is the Sample Collected?

A blood sample is drawn from a vein, usually in your arm. The serum (liquid part after clotting) is then analyzed for protein composition.

What Are Common Reasons for Testing?

Common reasons include evaluating symptoms like swelling, unexplained weight loss, or signs of organ dysfunction, as well as monitoring chronic conditions.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance: How Results May Affect Your Health

Short name

Total Protein Profile

Frequently asked questions

How is the test performed?
This test is typically performed using serum separated from blood through centrifugation. The protein fractions are analyzed via electrophoresis, which separates proteins based on size and charge. Results may be reported as total protein levels and relative proportions of albumin and globulins.
What do low or high protein levels usually indicate?
Low total protein or low albumin levels may suggest malnutrition, liver disease, kidney problems, or severe infections. High globulin levels could indicate chronic inflammation, autoimmune disorders, or certain cancers.
Can diet affect these results?
Yes, diet can influence protein levels. High-protein diets or deficiencies in protein intake may alter results. Always follow your doctor’s guidance on fasting or dietary restrictions before testing.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy