Skip to content

24-Hour Urinary Testosterone Level (Mass Concentration)

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of testosterone found in your urine over a 24-hour period. Testosterone is a hormone involved in male and female development, as well as sex drive and muscle mass.

Quick summary

["This test helps assess hormone balance and may be used in conditions related to hormone production or metabolism."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
15080-5
Short code
LH 24h Ur
Synonyms
LH 24h Ur
Category
chemistry
Sample
urine
Container
Sterile urine container
Units
N/A
Ref low
1.5
Ref high
9
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
83002
Specimen volume
10 mL
Stability
Room temperature: 2h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

Why This Test Might Be Ordered

Your healthcare provider may recommend this test if you have symptoms or conditions linked to hormone imbalances, such as low libido, infertility, or unexplained changes in body hair or voice. It could also be part of an evaluation for conditions like polycystic ovary syndrome (PCOS) or androgen-related disorders.

What to Expect Before the Test

You’ll need to collect all urine produced in a 24-hour period (from one morning to the next morning) in a special container. Drink plenty of water to help flush hormones into your urine. Avoid certain medications or supplements that might affect hormone levels, as directed by your provider.

How the Test Works

The collected urine is analyzed in a lab to measure the total amount of testosterone excreted over the 24-hour period. This provides a more comprehensive picture of your body’s testosterone production compared to a single blood test.

What the Results Mean

Results are typically compared to reference ranges for age and sex, though exact values may vary by lab. High levels might suggest conditions like Cushing’s syndrome or tumors, while low levels could indicate hormonal deficiencies or other health issues.

Reference range

Follicular: 1.5-9.0 mIU/mLN/A

Specimen: urine

For females: inform if menstrual cycle status. For certain tests, timing within menstrual cycle may matter.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

24h Urine Testosterone

Frequently asked questions

What does a high testosterone level in urine mean?
High urinary testosterone levels may indicate overproduction of testosterone, which can be linked to conditions like Cushing’s syndrome, adrenal tumors, or certain cancers. It may also suggest excessive androgen exposure, though further evaluation is needed to determine the cause.
What does a low testosterone level in urine mean?
Low urinary testosterone levels may signal hormonal imbalances, such as hypogonadism (low testosterone production), pituitary disorders, or conditions affecting hormone metabolism. This could contribute to symptoms like fatigue, low libido, or muscle weakness.
How often should I get this test?
This test is usually ordered as needed based on your symptoms or medical concerns. Your provider will determine if repeat testing is necessary, especially if treatment or monitoring for a specific condition is involved.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy