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Luteinizing Hormone (LH) and Prolactin (PRL) Profile with Follicle-Stimulating Hormone (FSH) and Estradiol (E2) Quantification in Serum/Plasma

Published: August 9, 2026Updated: August 16, 2026

This test measures levels of hormones involved in reproductive health, including LH, FSH, PRL, and estradiol, typically drawn from a blood sample to assess hormone balance.

Quick summary

["This profile helps evaluate fertility, hormone disorders, and reproductive function."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
40378-2
Short code
LH pre Triple Bolus SerPl-aCnc
Synonyms
LH pre Triple Bolus SerPl-aCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
1.5
Ref high
9
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
83002
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What This Test Measures

The test includes four key hormones: Luteinizing Hormone (LH), Follicle-Stimulating Hormone (FSH), Prolactin (PRL), and Estradiol (E2). These hormones play critical roles in regulating menstrual cycles, ovulation, and reproductive health in both men and women.

Why It’s Ordered

Doctors may order this test to diagnose or monitor conditions such as infertility, hormonal imbalances, polycystic ovary syndrome (PCOS), thyroid disorders, or pituitary issues. It can also be used during fertility treatments or before certain surgeries.

How the Test Works

A blood sample is drawn, typically from a vein in your arm. The sample is then analyzed in a lab to measure hormone concentrations using sensitive immunoassay methods.

What to Expect Before the Test

For women, timing can matter. Some tests may require a specific menstrual cycle phase or fasting (no food for 8–12 hours before). Follow your healthcare provider’s instructions to ensure accurate results.

Reference range

Follicular: 1.5-9.0 mIU/mLN/A

Specimen: serum

For females: inform if menstrual cycle status. For certain tests, timing within menstrual cycle may matter.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

LH/PRL/FSH/E2 Profile

Frequently asked questions

Why might my hormone levels be checked?
Your doctor may recommend this test if you have symptoms like irregular periods, infertility, unexplained weight changes, or signs of hormonal imbalances. Elevated or low hormone levels can indicate conditions like thyroid disorders, PCOS, or pituitary tumors.
How long will I need to wait for results?
Results are usually available within 1–3 business days, depending on your lab. Your healthcare provider will discuss them with you during a follow-up visit.
Can I take anything before the blood draw?
Yes, follow your doctor’s instructions carefully. Some tests require fasting, while others may need you to avoid certain medications or activities for a day or two before the test.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy