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Serum/plasma free testosterone concentration (mass concentration)

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of free (unbound) testosterone in your blood, which is an important hormone for men and women. Free testosterone is the biologically active form that directly affects sex drive, muscle mass, and overall health.

Quick summary

["Understanding your free testosterone levels can help assess hormone balance and potential health risks."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
27851-5
Short code
LH sp3 p chal SerPl-aCnc
Synonyms
LH sp3 p chal SerPl-aCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
1.5
Ref high
9
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
83002
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why Is This Test Done?

Your healthcare provider may order a free testosterone test to evaluate symptoms related to hormone imbalances, such as low libido, fatigue, or changes in body hair. It’s also used in fertility evaluations, hormone replacement therapy planning, or monitoring conditions like polycystic ovary syndrome (PCOS) or hypogonadism.

What to Expect Before the Test

No special preparation is needed for this blood test. You may be asked to fast for 8–12 hours before drawing blood, but this depends on your provider’s instructions. Avoid smoking or consuming alcohol for at least 24 hours before testing.

What Happens During the Test?

A healthcare professional will draw blood from a vein in your arm. The blood will be sent to a lab for analysis. The test typically takes a few minutes, and you can resume normal activities afterward.

What Are the Results?

Results are reported as a mass concentration (measured in picograms per milliliter, pg/mL). Your provider will compare these results to your individual reference range and clinical context to interpret them.

Reference range

Follicular: 1.5-9.0 mIU/mLN/A

Specimen: serum

For females: inform if menstrual cycle status. For certain tests, timing within menstrual cycle may matter.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Free testosterone levels can help diagnose or monitor conditions affecting hormone function. Low free testosterone may indicate hypogonadism, while high levels could suggest conditions like Cushing’s syndrome or androgen excess. It’s particularly useful in diagnosing fertility issues or evaluating symptoms of hormone-related disorders.

Short name

Free Testosterone

Frequently asked questions

What Does a Low Free Testosterone Level Mean?
Low free testosterone may indicate conditions like hypogonadism (low testosterone production), pituitary disorders, or certain medications that affect hormone levels. Symptoms may include reduced sex drive, fatigue, or muscle weakness. Your provider will discuss next steps based on your overall health and symptoms.
What Does a High Free Testosterone Level Mean?
High free testosterone can be caused by conditions like PCOS, tumors, or excessive androgen production. It may lead to symptoms like acne, irregular periods (in women), or excessive hair growth. Your provider will work with you to determine the cause and recommend appropriate treatment.
How Often Should I Get This Test?
The frequency depends on your medical condition and symptoms. Your provider may recommend regular monitoring if you have a known hormone-related disorder, are undergoing treatment, or have risk factors like obesity or genetic conditions. Follow-up tests are usually scheduled based on your individual care plan.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy