Genotypic Resistance Testing for Protease Inhibitors (Lopinavir)

Why This Test Is Done

Your healthcare provider may order this test if you’re taking lopinavir for HIV treatment and show signs of treatment failure, such as persistent or worsening viral load despite therapy. It helps identify specific genetic mutations in the virus that could reduce the effectiveness of lopinavir.

How the Test Works

A small sample of your blood is taken to analyze viral genetic material. Advanced laboratory techniques (like high-performance liquid chromatography or sequencing) identify mutations in the protease gene that may affect lopinavir’s effectiveness. Results help guide whether to switch medications or adjust treatment.

Sample Collection and Preparation

Blood is drawn from a vein, typically in your arm. The sample is processed to extract viral RNA/DNA, which is then analyzed for resistance-associated mutations. This test is usually done on fresh or stored blood samples.

What to Expect Before the Test

No special preparation is needed, but inform your provider if you’re taking medications that could affect blood clotting or if you have bleeding disorders. Avoid alcohol before the draw if possible.