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Left Retina Retinal Nerve Fiber Layer (RNFL) Thickness Test, Clock Hour 6, by Optical Coherence Tomography (OCT)

Published: August 9, 2026Updated: August 17, 2026

A non-invasive imaging test that uses safe, low-intensity light waves to measure the thickness of the retinal nerve fiber layer at clock hour 6 in your left eye, helping your eye doctor evaluate the health of the optic nerve and detect early signs of glaucoma, optic neuritis, or other conditions that may affect your vision.

Quick summary

{"Uses light waves to create detailed cross-sectional images of the retina","Helps detect and monitor glaucoma and other optic nerve diseases","The test is quick, painless, and requires no blood sample","Measures thickness at specific clock hours around the optic disc","Results help doctors track changes in eye health over time"}

Medically reviewed by WellAlly Editorial TeamLast reviewed August 17, 2026Editorial policy

Key facts at a glance

LOINC
86298-7
Short code
RNFL-L-6
Synonyms
L ret RNFL 6 o'clock thickness OCT
Category
cardiac
Sample
serum
Container
N/A - This is an imaging test not requiring specimen collection
Units
N/A
Ref low
100
Ref high
140
Critical range
— – 10000 N/A
Fasting
false
Population
adult
Sex
all
Age range
≥ 18 years
Method
Optical Coherence Tomography (OCT)
CPT
92134
SNOMED
373741007
Specimen volume
N/A - Imaging study
Stability
N/A - Imaging study
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
24–48 hours
Order
routine

What is this test?

The Left Retina Retinal Nerve Fiber Layer (RNFL) thickness test by OCT is a specialized imaging procedure that measures the thickness of the nerve fiber layer surrounding the optic nerve in your left eye. Optical Coherence Tomography (OCT) uses light waves to capture detailed cross-sectional images of the retina, similar to how ultrasound uses sound waves. This test focuses specifically on clock hour 6, which corresponds to the inferior portion of the optic nerve.

How is the test performed?

During the test, you will sit in front of the OCT machine and rest your chin on a support. The technician will ask you to look at a specific target while the machine scans your eye. The process is completely painless and takes only a few minutes. No eye drops or blood samples are required. The device uses low-intensity light to create high-resolution images of your retinal layers.

Why is it important?

The retinal nerve fiber layer consists of nerve fibers that carry visual information from the retina to the brain. Measuring its thickness helps doctors detect early changes that may indicate glaucoma, optic neuritis, or other conditions affecting the optic nerve. Comparing results over time helps track disease progression or treatment effectiveness.

Reference range

Normal Retinal Nerve Fiber Layer (RNFL) thickness at clock hour 6 (inferior temporal) typically ranges from 100-140 micrometers (μm). Values below 100 μm suggest focal RNFL thinning which may indicate glaucomatous or neurologic damage.N/A

Specimen: serum

Patient should remove contact lenses prior to imaging. Pupillary dilation may be performed for better visualization but is not always required. Patient should be informed that the test involves bright light exposure and should avoid rubbing eyes immediately before the scan.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

This test is primarily used for detecting and monitoring glaucoma, a condition that damages the optic nerve and can lead to vision loss if untreated. Thinning of the retinal nerve fiber layer, particularly at clock hour 6 (the inferior quadrant), may indicate glaucomatous damage. The test also helps differentiate between various optic nerve disorders and can detect damage from conditions like optic neuritis, ischemic optic neuropathy, or toxic/nutritional optic neuropathies. Early detection allows for timely intervention to preserve vision.

Frequently asked questions

Does the test hurt?
No, the OCT test is completely painless. You will only feel the need to sit still for a few moments while the images are captured. The light used is very dim and safe for your eyes.
How long does the test take?
The actual scanning process takes only about 1-2 minutes per eye. The entire appointment, including preparation, typically lasts 10-15 minutes.
Do I need to prepare for the test?
No special preparation is needed. You can eat and drink normally before the test. However, you should avoid wearing eye makeup on the day of the test as it may interfere with the imaging.
When will I get my results?
Your doctor will typically discuss the results with you during a follow-up appointment, usually within a week of the test. The images need to be analyzed by your eye care specialist.
How often should I have this test?
The frequency depends on your eye condition. For glaucoma patients, testing may be done annually or more frequently to monitor disease progression. Your doctor will recommend an appropriate schedule based on your individual situation.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 17, 2026. · Read our editorial policy