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Serum or Plasma Substance Concentration by Immunoassay (TMB-based)

Published: August 9, 2026Updated: August 16, 2026

This test measures a specific protein or substance in your blood using a sensitive immunoassay method that detects changes in color (tetramethylbenzidine, or TMB). It helps identify levels of certain markers that may indicate health conditions or responses to treatments.

Quick summary

["This test detects specific proteins in your blood using a color change to measure their presence and concentration."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
23213-2
Short code
LSDV Ab Titr Ser IF
Synonyms
LSDV Ab Titr Ser IF
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
if
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What This Test Measures

The test uses an immunoassay technique to detect and quantify a particular substance in your blood. The method relies on antibodies binding to the target analyte, triggering a chemical reaction that produces a measurable color change (TMB). This helps determine whether the substance is present above or below expected levels.

How the Test Works

Your blood sample is processed to separate serum or plasma, where the target substance is isolated. The immunoassay reagent is added, and if the substance is present, it reacts with the antibodies to produce a detectable signal. The color intensity correlates with the concentration of the analyte.

Common Uses

This test may be used to screen for, diagnose, or monitor conditions related to specific proteins, such as inflammation markers, autoimmune diseases, or certain infections. It can also assess drug levels or therapeutic responses in some cases.

When You Might Need This Test

Your healthcare provider may order this test if you have symptoms suggestive of a condition involving the target analyte, such as unexplained fevers, chronic inflammation, or suspected autoimmune activity. It may also be part of routine monitoring for certain treatments.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

TMB-based Immunoassay (Serum/Plasma)

Frequently asked questions

Why might my test results be important?
Elevated or altered levels of the measured substance may signal underlying health issues, such as infections, immune system disorders, or other metabolic conditions. Your provider will interpret the results in the context of your symptoms, medical history, and other test results to guide diagnosis or treatment planning.
What does a normal or abnormal result mean?
While specific reference ranges vary by analyte, abnormal results may prompt further testing or adjustments to your treatment plan. For example, very high or low levels could indicate a need for additional evaluation or intervention.
How long will it take to get my results?
Results are typically available within 24 to 48 hours, depending on lab processing times and your healthcare provider’s workflow. Follow-up instructions will be provided by your provider once results are ready.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy