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Lymphocyte Subset Analysis (with Flow Cytometry)

Published: August 9, 2026Updated: August 16, 2026

This test measures different types of white blood cells called lymphocytes in your blood, which help your immune system fight infections and diseases. It can help doctors understand how your immune system is working or identify problems with specific lymphocyte types.

Quick summary

["This test helps assess immune function and identify potential immune disorders."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
32562-1
Short code
Lymphs IgG/lymph NFr Spec
Synonyms
Lymphs IgG/lymph NFr Spec
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
82784
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Doctors may order this test if you have symptoms like frequent infections, unusual fatigue, or if you’re being treated for a condition that affects your immune system. It can also be used before certain treatments, such as chemotherapy, to check how your immune cells respond.

What to Expect Before the Test

No special preparation is needed. You’ll have a small needle inserted into a vein (usually in your arm), and blood will be drawn into a tube. The process takes about 5 minutes, and you may feel a slight pinch. If you’re nervous, let your doctor or nurse know.

What Happens During the Test

The blood sample is sent to a lab where it’s analyzed using a special machine called a flow cytometer. This machine separates and identifies different types of lymphocytes based on their size, shape, and surface markers. Results are usually ready within 24 hours.

What the Results Mean

The test reports percentages or counts of different lymphocyte types, such as T cells, B cells, and natural killer (NK) cells. Abnormal results may suggest conditions like immune deficiencies, autoimmune diseases, or certain cancers. Your doctor will interpret the results in the context of your health and symptoms.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Abnormal lymphocyte counts can indicate immune system disorders, such as low immunity (e.g., HIV/AIDS), overactive immune responses (e.g., lupus or rheumatoid arthritis), or conditions like leukemia or lymphoma. This test helps guide treatment decisions and monitor immune health over time.

Short name

Lymphocyte Profile

Frequently asked questions

What does a ‘lymphocyte profile’ tell me about my immune system?
It provides detailed information about the types and numbers of lymphocytes in your blood, helping doctors see if your immune system is functioning normally or if certain cell types are too high or too low. For example, low T cells might suggest HIV progression, while high B cells could point to an autoimmune condition.
How long does it take to get my results?
Most results are ready within 24 hours, but some labs may take up to a few days. Your doctor will contact you once they have the results, especially if they need further review.
Can I eat or drink before this test?
No special diet or fasting is required for this test. You can eat and drink normally before your blood draw, as it’s not affected by food or hydration.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy