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Lymphocyte Subset Analysis by Flow Cytometry (Serum/Plasma)

Published: August 9, 2026Updated: August 16, 2026

This test measures different types of white blood cells called lymphocytes in your blood, which helps assess immune function and identify potential health concerns.

Quick summary

["Understanding your immune system’s balance of lymphocyte types can guide treatment for infections, autoimmune diseases, or immune deficiencies."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
17225-4
Short code
Lymphs SmIg/lymph NFr Bld
Synonyms
Lymphs SmIg/lymph NFr Bld
Category
chemistry
Sample
whole-blood
Container
Lavender-top (EDTA)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
3 mL
Stability
Room temperature: 4h, Refrigerated: 24h
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

Why This Test Is Done

Doctors may order this test to evaluate immune health, diagnose conditions like leukemia, lymphoma, or immune deficiencies, or monitor treatment responses for diseases like HIV or autoimmune disorders.

What to Expect Before the Test

No special preparation is needed. A blood sample is drawn from a vein, usually in your arm. You may feel a slight pinch, but it typically takes only a few minutes.

What the Results Mean

Results show proportions of different lymphocyte types (e.g., B cells, T cells, natural killer cells). Abnormal levels may indicate overactive or underactive immune responses or other health issues.

Common Conditions Monitored

This test helps diagnose or manage conditions such as chronic infections, immune system disorders (e.g., lupus, rheumatoid arthritis), or cancers affecting lymphocytes.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical significance: Abnormal lymphocyte counts or ratios can signal immune dysfunction, infections, or malignancies. For example, low T cells may indicate HIV progression, while high B cells could suggest autoimmune activity or certain cancers.

Short name

Lymphocyte Profile

Frequently asked questions

What does a ‘lymphocyte subset profile’ measure exactly?
It identifies specific types of lymphocytes (e.g., CD4+ T cells, B cells, or NK cells) using flow cytometry, which counts and categorizes these immune cells based on surface markers.
How long does it take to get results from this test?
Results are typically available within 24 to 48 hours, depending on lab processing times and whether additional testing is needed.
Can lifestyle changes affect lymphocyte counts?
Yes, stress, diet, sleep, and infections can temporarily influence lymphocyte numbers. However, chronic lifestyle factors may also impact long-term immune health, so discuss any concerns with your doctor.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy