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Lymphocyte Subset Phenotyping by Flow Cytometry (Peripheral Blood)

Published: August 9, 2026Updated: August 16, 2026

This test measures the types and proportions of white blood cells called lymphocytes in your blood, which helps assess immune function and identify potential immune disorders.

Quick summary

["Understanding your immune cell composition can help diagnose or monitor conditions like infections, autoimmune diseases, or immune deficiencies."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
90416-9
Short code
Lymph subset + B-cell phenotype Pnl Bld
Synonyms
Lymph subset + B-cell phenotype Pnl Bld
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Doctors may order this test to evaluate your immune system. It’s particularly useful for diagnosing conditions like leukemia, lymphoma, HIV/AIDS, or other immune-related disorders. It may also be used before or after treatments that affect the immune system, such as chemotherapy or organ transplants.

How the Test Works

A small sample of your blood is taken and analyzed using flow cytometry, a technique that uses light to identify and count different types of cells based on their unique surface markers. This helps distinguish between various lymphocyte subsets, such as T cells, B cells, and natural killer cells.

Sample Collection

Blood is drawn from a vein, usually in your arm, using a needle. You may feel a slight pinch, but the process is quick and typically done in a doctor’s office or lab.

Preparation and Results

No special preparation is needed for this test. Results are usually available within a few days and can help guide further medical decisions based on your immune health.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Abnormal lymphocyte subsets can indicate immune system dysfunction, such as an overactive immune response (e.g., autoimmune diseases), an underactive immune response (e.g., HIV/AIDS), or the presence of cancerous cells (e.g., lymphoma or leukemia). Doctors use these findings to tailor treatments and manage chronic conditions effectively.

Short name

Lymphocyte Subset Analysis

Frequently asked questions

What does a lymphocyte subset analysis tell me about my health?
It provides detailed information about the types and numbers of your immune cells, helping doctors assess whether your immune system is functioning normally or if there are signs of an infection, autoimmune disease, or other immune-related conditions.
How long does it take to get results from this test?
Results are typically available within 2 to 5 business days, depending on lab processing times. Your healthcare provider will discuss the findings with you during a follow-up visit.
Can I prepare for this test in any special way?
No special preparation is needed. However, inform your doctor about any medications or supplements you’re taking, as some may affect blood test results.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy