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Anti-MDA5 Antibody (Serum/Plasma) – Immunoassay

Published: August 9, 2026Updated: August 16, 2026

This test measures antibodies against the MDA5 protein, which may be linked to certain inflammatory or autoimmune conditions affecting the lungs or skin.

Quick summary

["This test helps diagnose rare inflammatory diseases like anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis or inflammatory myositis."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
103691-2
Short code
MDA5 Ab Ser IA-aCnc
Synonyms
MDA5 Ab Ser IA-aCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
ia
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What This Test Measures

The test detects the presence of antibodies (specifically anti-MDA5) in your blood. These antibodies target a protein called MDA5, which plays a role in immune responses. Elevated levels may indicate an autoimmune condition affecting muscle or lung tissues.

Why It’s Ordered

Your healthcare provider may order this test if you have symptoms such as lung inflammation, skin rashes, or muscle weakness, especially when other tests for common autoimmune diseases do not explain your condition.

Common Symptoms Linked to Positive Results

Positive results may be associated with symptoms like chronic lung inflammation (pneumonia-like illness), skin rashes (like dermatomyositis), or muscle pain. These symptoms can vary widely and may require further evaluation.

Sample Collection and Preparation

A blood sample is drawn from a vein in your arm. The sample is processed into serum or plasma for testing. Avoid alcohol or certain medications for 24 hours before testing, as they may affect results.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

Anti-MDA5 Antibody

Frequently asked questions

Anti-MDA5 antibodies are rare but strongly linked to specific autoimmune diseases, such as inflammatory myositis or pulmonary conditions. Early detection can help guide treatment and prevent complications.
This test is not typically used for routine screening. It is usually ordered when symptoms suggest a possible autoimmune or inflammatory condition related to MDA5 antibodies.
How long does it take to get results?
Results are usually available within 3 to 7 business days, depending on lab processing times. Follow-up with your healthcare provider to discuss the meaning of your results.
Can lifestyle changes affect this test?
While some medications or substances (like alcohol) may influence other tests, there is no strong evidence that lifestyle changes directly impact anti-MDA5 antibody levels. Always follow your provider’s instructions for preparation.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy