Anti-MDA5 Antibody (Serum) – Qualitative/Quantitative Line Blot Assay
This test detects the presence of antibodies against the MDA5 protein, which may help diagnose certain inflammatory or autoimmune conditions affecting the lungs or skin.
["This test may be used to identify rare inflammatory diseases linked to specific antibody patterns."]
Key facts at a glance
- LOINC
- 88725-7
- Short code
- MDA5 Ab Ser Line blot-aCnc
- Synonyms
- MDA5 Ab Ser Line blot-aCnc
- Category
- chemistry
- Sample
- serum
- Container
- Red-top tube (SST)
- Units
- N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- Method
- line-blot
- Specimen volume
- 5 mL
- Stability
- Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
- Hemolysis max
- 500
- Lipemia max
- 1000
- Icterus max
- 500
- TAT
- 4–24 hours
- Order
- routine
What This Test Measures
The MDA5 protein is involved in immune responses, particularly in detecting viral infections. When the body produces antibodies against MDA5, it may signal an autoimmune reaction or an inflammatory process. This test uses a line blot assay to identify these antibodies in your serum sample.
Why It’s Ordered
Your healthcare provider may order this test if you have symptoms like lung inflammation (e.g., interstitial lung disease), skin rashes (e.g., dermatomyositis), or unexplained fatigue. It’s particularly useful for diagnosing rare conditions like anti-MDA5-associated interstitial lung disease (AMILD).
Sample Collection and Processing
A blood sample is drawn from a vein, typically in the arm. The serum portion is separated and tested for antibody presence using a specialized line blot assay, which detects specific protein targets.
What to Expect Before the Test
No special preparation is needed, but inform your provider if you’re taking blood thinners or have a history of bleeding disorders. Avoid alcohol or strenuous activity 24 hours before testing.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
Clinical Significance
Short name
Anti-MDA5 Antibody (Serine Blot)
Frequently asked questions
Anti-MDA5 antibodies are strongly linked to a specific subtype of interstitial lung disease (AMILD) and dermatomyositis. Positive results may indicate an autoimmune response requiring further evaluation, including imaging or lung function tests, to guide treatment decisions.
How long will results take? Results are typically available within 3–7 business days, depending on lab processing times.
Can I fast before this test? No fasting is required, but hydration is encouraged.
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy