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Anti-MDA5 Antibody (Serum) by Line Blot Immunoassay

Published: August 9, 2026Updated: August 16, 2026

This test detects antibodies against the MDA5 protein, which may be linked to certain inflammatory or autoimmune conditions. Results help diagnose rare connective tissue diseases.

Quick summary

["This test may help identify conditions like limited cutaneous systemic sclerosis (lcSSc) or other autoimmune disorders involving MDA5 antibodies."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
82424-3
Short code
MDA5 Ab Ser Ql Line blot
Synonyms
MDA5 Ab Ser Ql Line blot
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
line-blot
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What Is MDA5 and Why Does It Matter?

MDA5 (Melanoma Differentiation-Associated Protein 5) is a protein involved in the body’s immune response to viral infections and certain inflammatory processes. Antibodies against MDA5 can indicate an autoimmune reaction, particularly in conditions like limited cutaneous systemic sclerosis (lcSSc), where the skin and internal organs are affected.

How Is This Test Performed?

A blood sample is drawn and tested using a line blot immunoassay to detect the presence of anti-MDA5 antibodies. This method helps confirm or rule out specific autoimmune conditions based on antibody presence.

What Conditions Might This Test Be Used For?

This test is primarily used to screen for or confirm limited cutaneous systemic sclerosis (lcSSc) and other rare autoimmune disorders where MDA5 antibodies are associated with disease activity.

What Does a Positive Result Mean?

A positive result suggests the presence of anti-MDA5 antibodies, which may indicate an autoimmune response. Further evaluation by a healthcare provider is needed to assess symptoms and guide treatment.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Anti-MDA5 Antibody

Frequently asked questions

Why might my doctor order this test?
Your doctor may recommend this test if you have symptoms of autoimmune disease, such as skin changes, joint pain, or unexplained inflammation, particularly if they suspect conditions like systemic sclerosis or related disorders.
What are the typical symptoms linked to anti-MDA5 antibodies?
Symptoms may include skin thickening (scleroderma), lung inflammation (pneumonitis), muscle weakness, or fatigue. However, symptoms can vary widely and may not directly correlate with test results alone.
Do I need to prepare for this test?
No special preparation is needed. You may have a blood draw at your doctor’s office or a lab visit, and results will be reviewed by your healthcare team.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy