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Protein Electrophoresis (Serum/Plasma) with Bromocresol Green (BCG) Dye Binding Assay

Published: August 9, 2026Updated: August 16, 2026

This test measures the distribution of proteins in your blood, including albumin and globulins, using a dye-binding method to assess protein quality and potential abnormalities.

Quick summary

["Helps identify protein patterns that may indicate conditions like liver disease, kidney problems, or certain infections."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
95172-3
Short code
M iowae DNA Ct Spec Qn NAA+probe
Synonyms
M iowae DNA Ct Spec Qn NAA+probe
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
probe-amp-tar
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What This Test Measures

Protein electrophoresis separates blood proteins into fractions (e.g., albumin, alpha, beta, gamma globulins) based on size and charge. The BCG dye binds to proteins, allowing measurement of their concentration. This helps detect shifts in protein levels that may signal underlying health issues.

Common Uses

This test is often used to diagnose conditions affecting protein synthesis or breakdown, such as liver disease (e.g., cirrhosis), kidney disease, or immune system disorders. It may also be part of a broader evaluation for unexplained symptoms like fatigue, swelling, or unexplained weight changes.

How the Test Works

Blood is drawn and processed to separate proteins. The BCG dye binds to albumin and globulins, and the test measures how much dye is bound to each protein fraction. Results are compared to a reference pattern to identify abnormal shifts.

When You Might Need This Test

Your healthcare provider may recommend this test if you have symptoms like unexplained weight loss, swelling in limbs, frequent infections, or signs of liver/kidney disease. It may also be part of routine monitoring for certain conditions.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

BCG Protein Electrophoresis

Frequently asked questions

Why might my doctor order this test?
Your doctor may order this test to investigate protein-related disorders, such as hypoalbuminemia (low albumin), abnormal globulin patterns (e.g., monoclonal gammopathy), or conditions affecting protein metabolism. It can also help monitor treatment responses in patients with chronic diseases.
What do abnormal results mean?
Abnormal results may indicate conditions like liver disease (reduced albumin), immune disorders (elevated globulins), or kidney disease (protein loss in urine). Always discuss results with your healthcare provider, as interpretation depends on your overall health and symptoms.
How long will it take to get results?
Results are typically available within 24–48 hours, depending on your lab’s processing schedule. Your doctor will review them and discuss findings with you during your follow-up visit.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy