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Line Blot Immunoassay for Antibodies to Borrelia burgdorferi (Lyme Disease)

Published: August 9, 2026Updated: August 16, 2026

This test checks for antibodies your body makes in response to *Borrelia burgdorferi*, the bacterium responsible for Lyme disease. It helps determine if you’ve been exposed to the infection.

Quick summary

["A simple blood test to detect potential Lyme disease exposure."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
82426-8
Short code
Mj Ab Ser Ql Line blot
Synonyms
Mj Ab Ser Ql Line blot
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
line-blot
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

How the Test Works

Your blood sample is tested using a line blot immunoassay, which detects specific antibodies against *Borrelia burgdorferi* proteins. This method identifies whether your immune system has recognized the bacteria, even if symptoms haven’t yet appeared.

What to Expect Before the Test

No special preparation is needed. Bring your medical records if you’ve had prior Lyme disease testing or symptoms. Wear comfortable clothing and fast for 8–12 hours if fasting is required (though not always applicable for this test).

What the Results Mean

Results are qualitative (positive, negative, or inconclusive). A positive result suggests exposure but doesn’t confirm Lyme disease—further testing (like Western blot) is often needed. A negative result means no detectable antibodies were found at this time.

When You Might Need This Test

Doctors order this test if you’ve had tick bites, flu-like symptoms, joint pain, or rash (erythema migrans) in areas where Lyme disease is common. It’s also used in follow-up for people with suspected Lyme disease or recurrent symptoms.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

Lyme Disease Antibody Screening

Frequently asked questions

Why is this test important?
Early detection of Lyme disease antibodies helps guide treatment decisions. While antibodies may take weeks to develop after exposure, this test can flag potential risk, allowing for prompt evaluation and potential antibiotic therapy if needed.
What does a positive result mean?
A positive result indicates your immune system has likely encountered *Borrelia burgdorferi*, but it doesn’t confirm Lyme disease without additional testing. Symptoms may still appear days to months later, so consult your doctor for further evaluation.
Can I get this test at home?
No, this test is performed in a clinical lab. A healthcare provider will collect your blood sample during a visit to a doctor’s office or lab.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy