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Immunoassay for Anti-Nuclear Antibodies (ANA) using a probe-based method (qualitative screening)

Published: August 9, 2026Updated: August 16, 2026

This test measures antibodies your immune system may produce against parts of your own cells, often linked to autoimmune conditions.

Quick summary

["ANA tests help identify potential autoimmune diseases by detecting unusual antibodies in your blood."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
45117-9
Short code
M kansasii rRNA Spec Ql NAA+probe
Synonyms
M kansasii rRNA Spec Ql NAA+probe
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
probe-amp-tar
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What Does This Test Measure?

The test detects the presence of anti-nuclear antibodies (ANA), which target components of the cell nucleus. These antibodies can be found in people with autoimmune diseases, such as lupus or rheumatoid arthritis, though they can also occur in healthy individuals without symptoms.

Why Might You Need This Test?

Your healthcare provider may order this test if you have symptoms like joint pain, fatigue, rash, or other signs of an autoimmune condition. It can also be used as part of a routine checkup for certain risk factors or conditions.

What to Expect Before the Test

No special preparation is usually needed. You may be asked to fast for 8–12 hours before the blood draw if your lab requires it, but this is not always the case for ANA testing. Bring any medications you’re currently taking to your appointment.

What Happens During the Test?

A healthcare professional will draw blood from a vein in your arm. You may feel a slight pinch, but the process is quick and usually painless. The blood sample is then sent to a lab for analysis using an immunoassay method to detect ANA antibodies.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

ANA Screening Test

Frequently asked questions

What Does a Positive or Negative Result Mean?
A positive ANA test does not definitively diagnose an autoimmune disease. It may indicate a higher risk, prompting further testing (like specific antibody panels or other bloodwork) to narrow down possible conditions. A negative result means no detectable ANA antibodies were found, but it does not rule out autoimmune disease entirely.
Can This Test Be Used to Monitor Treatment?
Sometimes, ANA levels can be monitored over time if you’re being treated for an autoimmune condition. However, ANA tests alone are not typically used for ongoing monitoring because their results can fluctuate and lack specificity. Your doctor may use other tests or clinical symptoms for follow-up.
What Are Common Causes of False Positives or Negatives?
False positives can occur due to infections, certain medications, or other health issues. False negatives are less common but may happen if the test was not performed correctly or if the antibodies were present at very low levels. If your results are unclear, your doctor may repeat the test or order additional tests.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy