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Serum or Plasma Protein Electrophoresis (Qualitative) with Bromocresol Green/Blue Staining

Published: August 9, 2026Updated: August 16, 2026

This test measures the distribution of different protein types in your blood, often used to identify unusual patterns that may signal conditions like multiple myeloma, liver disease, or other protein-related disorders.

Quick summary

["This test helps doctors check for abnormal protein levels that could indicate hidden health issues."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
96272-0
Short code
MLH3 Ag Ca spec Ql ImStn
Synonyms
MLH3 Ag Ca spec Ql ImStn
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
1.5
Ref high
9
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
immune-stain
CPT
83002
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What This Test Measures

Protein electrophoresis separates blood proteins into fractions (e.g., albumin, globulins) based on size and charge. Bromocresol dyes (green/blue) bind to specific protein groups, revealing their relative amounts. This qualitative method doesn’t provide exact quantities but highlights shifts in protein composition.

Why It’s Done

Your doctor may order this test if you have symptoms like unexplained weight loss, fatigue, swollen lymph nodes, or signs of organ dysfunction (e.g., liver or kidney). It’s also used as a screening tool for conditions like monoclonal gammopathy or systemic diseases affecting protein synthesis.

Sample Collection and Preparation

A blood sample is drawn from a vein, usually in your arm. The serum or plasma is then processed and stained with bromocresol dye. Results are interpreted visually by comparing patterns to standard references.

What to Expect Before the Test

No special preparation is needed, but inform your doctor about medications (e.g., blood thinners) or recent illnesses that might affect protein levels.

Reference range

Follicular: 1.5-9.0 mIU/mLN/A

Specimen: serum

For females: inform if menstrual cycle status. For certain tests, timing within menstrual cycle may matter.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Protein Electrophoresis (Qualitative)

Frequently asked questions

How long does it take to get results?
Results are typically available within 24–48 hours, depending on lab processing times. Your doctor will discuss findings during your follow-up visit.
Can I eat or drink before this test?
No special diet is required. However, avoid heavy meals or alcohol 24 hours before the blood draw to ensure accurate results.
What if my results are abnormal?
If your test shows unusual patterns, your doctor may order additional tests (e.g., bone marrow biopsy, further protein analysis) to investigate the cause. Follow-up may include lifestyle changes or targeted treatments based on your diagnosis.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy