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: Mycoplasma pneumoniae DNA, Nasopharyngeal Specimen, Qualitative Nucleic Acid Amplification Probe

Published: August 9, 2026Updated: August 16, 2026

: This test looks for the genetic material of Mycoplasma pneumoniae, a common bacterium that can cause respiratory infections, in a sample collected from the back of the nose and throat.

Quick summary

[": A positive result indicates that Mycoplasma pneumoniae genetic material was detected in the nasopharyngeal sample, suggesting an active infection."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
108164-5
Short code
M pneumo DNA Spec Ql NAA+n-prb
Synonyms
M pneumo DNA Spec Ql NAA+n-prb
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
non-probe-amp-tar
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

: What the Test Detects

: The test uses nucleic acid amplification, such as polymerase chain reaction (PCR), to detect a specific segment of DNA belonging to Mycoplasma pneumoniae. Because it identifies the organism's genetic material directly, it can indicate an active infection when present in a respiratory specimen.

: How the Sample Is Collected

: A nasopharyngeal specimen is collected using a swab inserted into the nasal passage to reach the area at the back of the nose and throat. This sample is then processed in the laboratory to extract and amplify any Mycoplasma pneumoniae DNA present.

: Why the Test May Be Ordered

: Your healthcare provider may order this test when you have symptoms of a respiratory infection, such as a persistent cough, fever, or sore throat, especially when a bacterial cause like Mycoplasma pneumoniae is suspected. It helps distinguish this infection from other causes of respiratory illness.

: What the Results Mean

: A positive result means Mycoplasma pneumoniae DNA was detected, supporting the diagnosis of an active infection. A negative result means the DNA was not detected, which generally suggests the organism is not the cause of your symptoms, though factors such as sample quality and timing can affect results. Your provider will interpret the result along with your symptoms and other findings.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

: Mycoplasma pneumoniae is a common cause of atypical pneumonia and upper respiratory infections, particularly in school-aged children and young adults. Nucleic acid amplification testing offers a sensitive and specific way to detect the organism early in infection, which can help guide appropriate treatment decisions, including whether antibiotics targeting this bacterium are warranted.

Short name

: M. pneumoniae PCR

Frequently asked questions

: Does a positive result always mean I have pneumonia?
: No. Mycoplasma pneumoniae can cause a range of respiratory illnesses, from mild upper airway infection to pneumonia. A positive result confirms the presence of the organism but your provider will determine the extent of illness based on your symptoms and examination.
: Can the test tell me if I am contagious?
: The test detects the presence of the organism's DNA but does not directly measure contagiousness. Mycoplasma pneumoniae can be spread through respiratory droplets, and your provider can advise on precautions based on your overall clinical picture.
: Do I need to fast or prepare before this test?
: Generally no special preparation is needed for a nasopharyngeal swab test. Follow any specific instructions given by your healthcare provider or the collection site.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy