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Serum Protein Electrophoresis (24-Hour Fractionated)

Published: August 9, 2026Updated: August 17, 2026

This test measures the different types of proteins in your blood, broken down by size and charge, to help identify abnormalities in protein distribution.

Quick summary

["Helps diagnose conditions like multiple myeloma, liver disease, or kidney problems by analyzing protein patterns in your blood."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 17, 2026Editorial policy

Key facts at a glance

LOINC
42484-6
Short code
M Protein 24h MFr Ur Elph
Synonyms
M Protein 24h MFr Ur Elph
Category
chemistry
Sample
urine
Container
Sterile urine container
Units
N/A
Ref low
7.35
Ref high
7.45
Critical range
7.2 N/A – 7.6 N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
electrophoresis
Specimen volume
10 mL
Stability
Room temperature: 2h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

What Does This Test Measure?

The test uses serum (blood plasma without clotting factors) to separate proteins into distinct bands based on their electrical charge and size. This helps identify which proteins are present in higher or lower amounts than expected.

Why Might You Need This Test?

Your doctor may order this test if you have symptoms like unexplained weight loss, fatigue, swelling, or if they suspect conditions like multiple myeloma, liver disease, or kidney disease that affect protein levels.

Common Conditions Monitored

This test can help detect or monitor conditions such as plasma cell dyscrasias (like multiple myeloma), chronic liver disease, nephrotic syndrome, or other protein-related disorders.

Sample Collection and Preparation

A blood sample is drawn from a vein, usually in your arm. The blood is then processed to separate serum, which is sent to the lab for electrophoresis. This process takes place within 24 hours of collection.

Reference range

NCBI: pH 7.35-7.45N/A

Specimen: urine

No special preparation usually required. Stay well hydrated. Inform your healthcare provider of all medications and supplements you are taking.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

Fractionated Serum Protein Electrophoresis

Frequently asked questions

How are protein patterns interpreted?
Abnormal patterns may show increased or decreased levels of specific proteins, such as globulins (which can indicate immune system issues) or albumin (which can signal liver or kidney problems). Your doctor will review the results in the context of your symptoms and medical history.
What do abnormal results mean?
Abnormal results may suggest conditions like multiple myeloma (where abnormal proteins called M proteins appear), liver disease (reduced albumin), or kidney disease (increased globulins). Follow-up tests or further evaluation may be needed to determine the exact cause.
How often is this test repeated?
The frequency depends on your condition and treatment plan. Your doctor will decide if you need follow-up tests, which could be monthly, quarterly, or annually depending on your situation.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 17, 2026. · Read our editorial policy