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Serum Protein Electrophoresis with Fractional Quantitation (Bromocresol Purple Method)

Published: August 9, 2026Updated: August 16, 2026

This test measures the different types of proteins in your blood, helping identify abnormalities in protein distribution that may signal conditions like kidney disease, liver problems, or certain cancers.

Quick summary

["This test helps diagnose and monitor conditions affecting protein balance in your blood."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
90990-3
Short code
M Protein isotype SerPl MS.MALDI-TOF
Synonyms
M Protein isotype SerPl MS.MALDI-TOF
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
ms-maldi-tof
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What Proteins Are Measured?

Electrophoresis separates blood proteins into groups based on size and electrical charge. The test quantifies key protein fractions, including albumin, alpha-globulins, beta-globulins, and gamma-globulins, which may reflect underlying health issues.

Why Is This Test Done?

Doctors may order this test to investigate symptoms like swelling, unexplained weight loss, fatigue, or to evaluate conditions like multiple myeloma, liver disease, or kidney disorders.

Sample Preparation and Testing Process

A blood sample is drawn and sent to a lab. Proteins are separated using an electric current, stained, and measured for concentration. The bromocresol purple method detects protein bands visually and quantifies their amounts.

Common Conditions Monitored

Abnormal results may indicate conditions such as hypoalbuminemia (low albumin), monoclonal gammopathy (unusual protein spikes), or signs of chronic inflammation or organ dysfunction.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Protein Electrophoresis

Frequently asked questions

What does a high or low protein result mean?
A high or low level of specific protein fractions can signal different health concerns. For example, low albumin might suggest malnutrition or kidney problems, while unusual spikes in certain globulins could indicate immune system issues or cancer.
How often should I get this test?
Your doctor will determine the frequency based on your health condition and treatment plan. Some patients need this test yearly or more often if managing chronic illnesses.
Can I eat or drink before this test?
You may have a light meal or drink water before the blood draw, but avoid heavy meals or alcohol for a few hours to ensure accurate results.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy