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Serum Protein Electrophoresis (SPE) with Bromocresol Purple Staining

Published: August 9, 2026Updated: August 17, 2026

This test measures the different types of proteins in your blood using a technique called electrophoresis. It helps identify abnormalities in protein distribution, which can indicate conditions like multiple myeloma, liver disease, or other disorders.

Quick summary

["This test helps doctors spot unusual protein patterns that may signal hidden health issues."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 17, 2026Editorial policy

Key facts at a glance

LOINC
33647-9
Short code
M Protein MFr SerPl Elph
Synonyms
M Protein MFr SerPl Elph
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
7.35
Ref high
7.45
Critical range
7.2 N/A – 7.6 N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
electrophoresis
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your doctor may order this test if they suspect conditions affecting protein levels, such as kidney disease, liver problems, or certain cancers. It’s also used to monitor treatment progress in conditions like multiple myeloma or to check for signs of chronic inflammation or infection.

How the Test Works

A small blood sample is taken and separated into different protein fractions based on size and charge. The bromocresol purple dye helps visualize these fractions when viewed under a microscope, showing how proteins are distributed across the sample.

Sample Collection and Preparation

You’ll have a needle inserted into your arm to draw blood. The sample is then processed and analyzed in a lab to separate proteins and detect any irregular patterns.

What to Expect Before the Test

No special preparation is needed, but inform your doctor about any medications you’re taking, as some may affect protein levels.

Reference range

NCBI: pH 7.35-7.45N/A

Specimen: serum

No special preparation usually required. Stay well hydrated. Inform your healthcare provider of all medications and supplements you are taking.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Protein Electrophoresis

Frequently asked questions

What Does an Abnormal Result Mean?
An abnormal result doesn’t always mean a serious condition, but it may signal a need for further testing. Your doctor will discuss findings with you and may recommend follow-up tests or treatments.
How Long Does It Take to Get Results?
Results are typically available within 1–3 business days, depending on lab processing times. Your doctor will contact you with the outcome.
Can I Eat or Drink Before the Test?
No special diet is required, but avoid heavy meals or alcohol before the blood draw to ensure accurate results.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 17, 2026. · Read our editorial policy