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Total Protein in Urine (24-Hour Collection)

Published: August 9, 2026Updated: August 16, 2026

This test measures the total amount of protein found in your urine over a 24-hour period. Protein in urine can sometimes indicate kidney problems or other health conditions.

Quick summary

["Protein in urine may signal kidney issues or other health concerns that need attention."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
101670-8
Short code
M Protein Ur Ql MS.MALDI-TOF
Synonyms
M Protein Ur Ql MS.MALDI-TOF
Category
chemistry
Sample
urine
Container
Sterile urine container
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
ms-maldi-tof
Specimen volume
10 mL
Stability
Room temperature: 2h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your healthcare provider may order this test if you have symptoms like swelling, frequent urination, or if they suspect kidney disease, diabetes, or other conditions that can affect protein levels in urine. It’s also used to monitor long-term kidney health or response to treatment.

What to Expect Before the Test

You’ll collect all urine for 24 hours in a special container. Drink plenty of water to help your kidneys produce urine, but avoid excessive caffeine or alcohol. Follow your provider’s instructions carefully to ensure accurate results.

How the Test Works

The collected urine is sent to a lab where it’s analyzed to measure the total protein content. The test may use methods like nephelometry or turbidimetry to detect protein levels.

What the Results Mean

Results are typically reported as the total protein amount in grams or milligrams over 24 hours. Higher than expected levels may indicate kidney problems, such as nephrotic syndrome or kidney damage. Lower levels may suggest dehydration or other conditions.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

24-Hour Urine Protein

Frequently asked questions

What does a high protein level in urine mean?
A high protein level in urine (proteinuria) can be a sign of kidney disease, where the kidneys aren’t filtering proteins properly. It may also occur with conditions like diabetes, high blood pressure, or certain autoimmune diseases. Early detection helps manage these conditions and prevent further damage.
What does a normal protein level in urine mean?
A normal protein level in urine usually means your kidneys are working well and filtering proteins as they should. However, even normal results shouldn’t replace regular check-ups with your healthcare provider, especially if you have risk factors for kidney issues.
How often should I get this test?
The frequency depends on your health status. If you have kidney disease, diabetes, or other conditions that may affect urine protein levels, your provider may recommend testing every few months or as needed. Follow their guidance for the best care plan.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy