Microbiome DNA Sequencing for Antibiotic Resistance Gene Profiling (from stool sample)

What This Test Measures

The test uses advanced DNA sequencing to identify the presence of genes (prokaryotic genetic material) that confer resistance to antibiotics in bacteria found in your stool. These genes may be carried by bacteria that live in your digestive system. The results help determine if certain antibiotics are likely to be effective or ineffective against your specific bacterial population.

Why It’s Ordered

Your healthcare provider may order this test if you have recurrent infections, symptoms suggesting bacterial overgrowth, or if antibiotics have not worked as expected. It’s also used in research or specialized care to monitor antibiotic resistance trends in your microbiome.

Sample Collection and Preparation

A stool sample is collected (typically via a bowel movement or stool collection kit) and sent to the lab. The lab processes the sample to extract DNA, which is then sequenced to detect antibiotic resistance genes. No special preparation is needed beyond standard stool collection guidelines.

Test Limitations and Considerations

This test focuses on genes, not the specific bacteria themselves. Results may not show all possible resistance mechanisms or detect viruses or fungi. It does not replace standard culture tests for identifying bacteria or guide treatment decisions alone.