Mycobacterium tuberculosis Complex (Mtb) Detection by Specimen Sequencing

What This Test Measures

The test looks for the DNA sequence of *Mycobacterium tuberculosis* (Mtb) and related species (Mtb complex). This includes identifying specific genetic markers that are unique to these bacteria. Sequencing is a highly sensitive method that can detect even small amounts of bacterial DNA in your sample.

Why It’s Done

Your healthcare provider may order this test if you have symptoms of tuberculosis (such as persistent cough, fever, weight loss, or chest pain) or if you’ve been exposed to someone with tuberculosis. It’s also used in screening, monitoring treatment response, or confirming a suspected infection in hard-to-diagnose cases.

Sample Requirements

The test requires a biological sample, such as **sputum, bronchoalveolar lavage fluid, or other respiratory secretions**, depending on clinical context. Sometimes, blood or other body fluids may be used if the infection is suspected elsewhere in the body.

How the Test Works

DNA from the bacteria is extracted from your sample and analyzed using high-throughput sequencing. The lab compares your sample’s genetic material to known Mtb sequences to confirm or rule out infection.