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Molecular Test for Detection of Mycobacterium tuberculosis DNA in Sputum

Published: August 9, 2026Updated: August 16, 2026

This test checks for genetic material from *Mycobacterium tuberculosis*, the bacteria responsible for tuberculosis (TB). It helps determine if you have an active TB infection by detecting its DNA in your sputum.

Quick summary

["A TB DNA test can help confirm or rule out TB infection faster than traditional cultures."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
107168-7
Short code
M tb DNA Tiss Ql NAA+probe
Synonyms
M tb DNA Tiss Ql NAA+probe
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
probe-amp-tar
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

How the Test Works

The test involves collecting a sputum sample (usually from coughing into a container) and analyzing it for TB bacteria DNA using advanced molecular techniques. This method is quicker than growing bacteria in a lab, often providing results in days rather than weeks.

Sample Collection

You’ll need to provide a sputum sample, typically from deep coughing. This is usually done in a healthcare setting where you’ll cough into a sterile container. Follow your doctor’s instructions carefully to ensure the sample is representative of your respiratory tract.

What to Expect Before the Test

No special preparation is usually needed, but you may be asked to avoid certain medications or foods that could interfere with the test. If you have a respiratory infection, your doctor may advise waiting until symptoms improve for the most accurate results.

When This Test Might Be Ordered

Your healthcare provider may recommend this test if you have symptoms of TB (like persistent cough, fever, or weight loss), have been exposed to TB, or if you live in an area with higher TB rates. It’s also used to monitor treatment progress or check for recurrence.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

TB DNA Sputum Test

Frequently asked questions

Why was I asked to take this test?
Your doctor may have recommended it due to symptoms like coughing, fever, or weight loss, exposure to TB, or if you live in a region with higher TB rates. It helps determine if you need treatment for TB infection.
What does a positive result mean?
A positive result means your sputum contains genetic material from *Mycobacterium tuberculosis*, strongly suggesting an active TB infection. This will guide your doctor in starting appropriate treatment.
How long will it take to get results?
Results are typically available within a few days to a week, depending on lab processing times. Your doctor will contact you with the results once they are ready.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy