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Serum or plasma mass concentration of vitamin D (25-hydroxyvitamin D)

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of vitamin D in your blood, which helps your body absorb calcium and maintain bone health. Low levels may indicate deficiency, which can affect muscle function, bone strength, and overall health.

Quick summary

["Vitamin D levels help prevent deficiencies that can lead to health risks like osteoporosis or muscle weakness."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
111023-8
Short code
MuV IgM Msmt Ser
Synonyms
MuV IgM Msmt Ser
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
82784
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why Is This Test Done?

Your healthcare provider may order this test if you have symptoms of vitamin D deficiency, such as fatigue, bone pain, or frequent infections. It’s also recommended for people at higher risk, like those with limited sun exposure, certain medical conditions (e.g., malabsorption), or older adults. Some tests may be done to monitor treatment for conditions like rickets or osteoporosis.

What to Expect Before the Test

No special preparation is usually needed, but you may be asked to fast for 8–12 hours before the test if it involves drawing blood from a vein. If you’re taking vitamin D supplements, let your provider know to avoid interfering with results.

What Happens During the Test?

A healthcare professional will insert a small needle into a vein in your arm to draw blood. You may feel a slight pinch, but it’s usually over quickly. The blood sample is then sent to a lab for testing.

What Are Possible Results?

Results are typically reported as a numerical value (e.g., micrograms per deciliter or nanograms per milliliter), but your provider will interpret them based on your health and symptoms. Very low or very high levels may require further evaluation.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Vitamin D deficiency is linked to weakened bones, muscle weakness, and an increased risk of certain chronic diseases. High levels may also cause symptoms like nausea, headache, or kidney stones, though this is rare. Your provider will discuss results in the context of your overall health and any symptoms you’re experiencing.

Short name

Vitamin D Level

Frequently asked questions

What causes vitamin D deficiency?
Vitamin D deficiency can result from limited sun exposure, poor diet, certain medical conditions (like celiac disease or Crohn’s), obesity, or medications that interfere with its absorption. Older adults may also produce less vitamin D naturally.
How long does it take to get results?
Blood test results are usually available within 1–3 business days, depending on your lab’s processing time. Your provider will contact you once they have the results.
Can I take vitamin D supplements if my levels are low?
Only follow your provider’s advice on supplements. They’ll determine the right dose and monitor you to avoid overuse, which can be harmful.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy