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Serum or plasma mass concentration of vitamin D (25-hydroxyvitamin D)

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of vitamin D in your blood, which helps your body absorb calcium and maintain bone health. Low levels may indicate deficiency, while high levels could signal overabsorption or certain medical conditions.

Quick summary

["Vitamin D levels help assess bone health and prevent deficiencies linked to conditions like osteoporosis or rickets."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
22418-8
Short code
MuV IgM Ser Ql
Synonyms
MuV IgM Ser Ql
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
82784
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why Is This Test Done?

Your doctor may order this test if you have symptoms like muscle weakness, bone pain, or if you’re at higher risk for vitamin D deficiency—such as living far from the equator, having limited sun exposure, or having certain medical conditions (e.g., malabsorption disorders). It’s also part of routine check-ups for older adults or those with chronic illnesses.

What to Expect Before the Test

No special preparation is needed, but you may be asked to fast for 8–12 hours before the draw if your lab requires it. Wear comfortable clothing and bring your medical records if you have any conditions that affect vitamin D levels.

During the Test

A healthcare provider will draw blood from a vein in your arm. You may feel a slight pinch, but the process is quick and usually painless. The blood sample is sent to a lab for analysis.

What the Results Mean

Results are reported in nanograms per milliliter (ng/mL) or picomoles per liter (pmol/L), but exact ranges depend on your lab. Low levels may prompt further testing or dietary/supplement adjustments, while very high levels could require medical evaluation.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Vitamin D deficiency is linked to weakened bones, muscle weakness, and increased infection risk. High levels may indicate over-supplementation or liver/kidney issues. Your doctor will interpret results in the context of your health history and symptoms.

Short name

Vitamin D level

Frequently asked questions

How long does it take to get my results?
Results are typically available within 1–3 business days, depending on your lab. Follow-up with your doctor if you have questions or concerns about your results.
What causes vitamin D deficiency?
Common causes include limited sun exposure, poor diet (low-fat fish, fortified foods), obesity, certain medications (e.g., anticonvulsants), or conditions like celiac disease or Crohn’s disease that affect nutrient absorption.
Can I take vitamin D supplements if my levels are low?
Yes, but only under medical supervision. Your doctor may recommend a specific dose and monitor your levels to avoid overuse. Never take high doses without guidance, as excess vitamin D can be harmful.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy