Skip to content

: Mycobacterium tuberculosis complex DNA, Sputum, Qualitative, Nucleic Acid Probe

Published: August 9, 2026Updated: August 16, 2026

: This test looks for genetic material (DNA) from the bacteria that cause tuberculosis in a sputum sample. It helps determine whether a person is infected with the tuberculosis bacteria.

Quick summary

[": A positive result suggests tuberculosis bacteria DNA is present in the sputum, while a negative result suggests it was not detected."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
14973-2
Short code
Mycobacterium DNA Spt Ql NAA+probe
Synonyms
Mycobacterium DNA Spt Ql NAA+probe
Category
chemistry
Sample
sputum
Container
Sterile sputum container
Units
N/A
Ref low
0.8
Ref high
1.2
Critical range
— – 5 N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
probe-amp-tar
CPT
85610
SNOMED
396252006
Specimen volume
3-5 mL
Stability
Room temperature: 2h, Refrigerated: 24h
TAT
4–24 hours
Order
routine

: What This Test Measures

: The test detects DNA belonging to the Mycobacterium tuberculosis complex, the group of bacteria responsible for tuberculosis. Because it targets genetic material, it can identify the bacteria directly rather than relying on the body's immune response, and results may be available sooner than traditional culture methods.

: Why It Is Ordered

: A healthcare provider may order this test when a person has symptoms such as a persistent cough, fever, night sweats, or weight loss, or when there has been known exposure to tuberculosis. It is often used together with imaging and other tests to help diagnose or rule out active tuberculosis.

: How the Sample Is Collected

: The test uses sputum, the mucus coughed up from the lungs. You will likely be asked to cough deeply and collect the sample in a sterile container, sometimes on more than one day. Follow the collection instructions carefully, since a good-quality sample improves the reliability of the result.

: What the Results Mean

: A detected result means tuberculosis bacteria DNA was found and supports further evaluation and public health reporting. A not-detected result means the DNA was not found, but it does not by itself always exclude tuberculosis, so your provider will interpret it alongside symptoms, imaging, and other tests. Results are reported as qualitative (detected or not detected) rather than as a number.

Reference range

INR: 0.8-1.2 (warfarin: 2.0-3.0)N/A

Specimen: sputum

Inform your healthcare provider if you are taking blood thinners. No special preparation required.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

: This nucleic acid probe test provides rapid, direct evidence of Mycobacterium tuberculosis complex in sputum and supports timely diagnosis and treatment decisions. A positive result generally warrants clinical correlation, additional testing such as culture and drug-susceptibility testing, and appropriate infection-control and public health follow-up. A negative result should be interpreted in the full clinical context, as it does not guarantee absence of infection.

Short name

: MTB DNA Probe

Frequently asked questions

: Is this the same as a TB skin test?
: No. A TB skin test measures the body's immune response to tuberculosis, whereas this test detects the bacteria's DNA directly in sputum. They answer different questions and are often used together, depending on the situation.
: Does a negative result mean I definitely do not have tuberculosis?
: Not necessarily. A negative result means the bacteria's DNA was not detected in that sample, but factors like sample quality or the amount of bacteria present can affect results. Your provider will combine the result with your symptoms, imaging, and other testing.
: Will I need other tests as well?
: Often yes. Results from this test are usually interpreted along with a chest X-ray, cultures, and sometimes drug-susceptibility testing, which helps guide the choice of treatment.
  1. 1
  2. 2
  3. 3

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy