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Serum Protein Electrophoresis (SPEP) with Bromocresol Green Dye Binding

Published: August 9, 2026Updated: August 16, 2026

This test measures the distribution of different protein types in your blood serum, often used to identify abnormalities in protein composition, such as those seen in conditions like multiple myeloma or liver disease.

Quick summary

["Helps detect protein abnormalities that may signal underlying health issues requiring further medical evaluation."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
68983-6
Short code
Nat (USA) PFGE pattern Islt
Synonyms
Nat (USA) PFGE pattern Islt
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your healthcare provider may order this test to investigate symptoms like unexplained weight loss, fatigue, or swelling, or to monitor conditions like kidney disease, liver disease, or certain cancers. It can also help diagnose or track diseases affecting protein levels in the blood.

How the Test Works

A small blood sample is drawn and separated into different protein fractions using electrophoresis. Bromocresol Green dye binds to specific proteins, allowing for visual differentiation of protein bands on a gel. This helps identify patterns or imbalances in protein distribution.

Sample Preparation and Testing Process

The test involves collecting serum from a blood draw, which is then processed and analyzed in a laboratory. The results are typically reported as a pattern or profile of protein bands, rather than specific numeric values.

What to Expect Before the Test

No special preparation is usually needed, but you may be asked to avoid certain medications or foods that could interfere with results. Fasted or non-fasted states may not affect this test significantly.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

SPEP with Bromocresol Green

Frequently asked questions

What does an abnormal result mean?
An abnormal result suggests there may be an imbalance in your blood proteins. This could point to conditions like cancer, liver or kidney problems, or other systemic diseases. Your doctor will discuss these findings with you and may recommend additional tests or treatments.
How often should I get this test?
The frequency depends on your health condition and medical needs. Some patients may need this test periodically to monitor their condition, while others may only need it once or twice for diagnostic purposes. Follow your healthcare provider’s instructions.
Can diet or medications affect this test?
Generally, diet does not significantly impact SPEP results. However, certain medications (like some steroids or chemotherapy drugs) might influence protein levels. Always inform your doctor about any medications you are taking before the test.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy