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Nicotinamide (Vitamin B3) Concentration in Serum or Plasma

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of nicotinamide, a form of vitamin B3, in your blood. Nicotinamide supports energy production and DNA repair.

Quick summary

["Nicotinamide helps your body function properly, especially in metabolism and cell health."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
56961-6
Short code
Nicotinamide SerPl-mCnc
Synonyms
Nicotinamide SerPl-mCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What Nicotinamide Does in Your Body

Nicotinamide is a water-soluble vitamin that plays a role in converting food into energy. It also helps maintain healthy skin, nerves, and the immune system. Your body produces some nicotinamide naturally, but you also get it from foods like meat, poultry, fish, and certain vegetables.

Why Your Doctor Might Order This Test

Your doctor may check your nicotinamide levels if you have symptoms like fatigue, skin issues, or digestive problems that could be linked to vitamin deficiencies. It can also be part of broader metabolic or nutritional assessments.

Potential Health Conditions

Low nicotinamide levels might be associated with conditions like pellagra (a rare but serious vitamin deficiency) or metabolic disorders. High levels could indicate overconsumption of supplements or certain medications.

Sample Collection and Preparation

This test requires a blood sample drawn from a vein in your arm. The blood is processed into either serum (after clotting) or plasma (anticoagulated). The sample is then sent to a lab for analysis using a specialized method, likely immunoassay or enzymatic techniques.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

Nicotinamide Level

Frequently asked questions

How might my nicotinamide levels affect my health?
Nicotinamide levels alone are less commonly monitored than other vitamins like B12 or folate, but they can provide clues about your overall nutritional status. If your levels are consistently low, your doctor may recommend dietary changes or supplements to address potential deficiencies.
Will I need to prepare for this test?
No special preparation is usually needed, but avoid heavy meals or alcohol before your blood draw to ensure accurate results. Your doctor or lab technician will provide specific instructions if required.
What does a low or high nicotinamide level mean?
Low levels might suggest poor diet, malabsorption issues, or certain medications affecting vitamin uptake. High levels could be due to excessive intake of supplements or medications containing nicotinamide. Your doctor will interpret results in the context of your overall health and symptoms.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy