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Nicotinamide (Vitamin B3) Metabolite Concentration (Urinary)

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of nicotinamide, a form of vitamin B3, found in your urine. It helps assess how your body processes and excretes this nutrient.

Quick summary

["This test may be used to evaluate vitamin B3 metabolism or detect certain metabolic conditions."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
3852-1
Short code
Nicotinamide Ur-mCnc
Synonyms
Nicotinamide Ur-mCnc
Category
chemistry
Sample
urine
Container
Sterile urine container
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
10 mL
Stability
Room temperature: 2h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

Why This Test Might Be Ordered

Your healthcare provider may recommend this test if they suspect a metabolic disorder affecting vitamin B3 metabolism, such as hereditary issues related to nicotinamide processing. It could also be part of a broader evaluation for conditions like Hartnup disease or other genetic disorders involving amino acid or vitamin transport.

What to Expect Before the Test

No special preparation is typically needed. You may be asked to collect a random urine sample, often during your regular urine collection period if part of a 24-hour study. Drink plenty of water to ensure adequate urine volume if instructed.

What Happens During the Test

A healthcare professional will collect a urine sample. The sample is sent to a lab for analysis using a specialized method to measure nicotinamide concentration. Results are usually reported as a mass concentration in urine.

What the Results Mean

Results are compared to reference ranges specific to the lab. Elevated levels might indicate certain metabolic disorders, while very low levels could suggest impaired vitamin B3 metabolism or other underlying conditions. Your provider will interpret results in the context of your overall health and symptoms.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Nicotinamide in urine

Frequently asked questions

What are the normal reference ranges for this test?
Reference ranges vary by lab and testing method. Your healthcare provider will use lab-specific values and your clinical picture to determine if results are within normal limits or indicate a concern.
Can I fast before this test?
No fasting is required for this test. However, follow any specific instructions your provider gives, such as avoiding certain medications or foods that might affect urine composition.
How often can I get this test?
This test is generally not routine and is typically ordered as needed based on clinical suspicion of a metabolic disorder. Your provider will determine the frequency based on your diagnosis and ongoing care.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy