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Nicotinamide (Vitamin B3) Concentration in Urine

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of nicotinamide, a form of vitamin B3, found in your urine. It may be used to assess vitamin B3 status or detect certain metabolic conditions.

Quick summary

["This test helps check if your body has enough vitamin B3 or if there may be an underlying metabolic issue."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
17362-5
Short code
Nicotinamide Ur Ql
Synonyms
Nicotinamide Ur Ql
Category
chemistry
Sample
urine
Container
Sterile urine container
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
10 mL
Stability
Room temperature: 2h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

Why This Test Might Be Ordered

Your healthcare provider may recommend this test if you have symptoms like fatigue, skin changes, or neurological issues that could be linked to vitamin B3 deficiency. It might also be part of a broader evaluation for conditions affecting metabolism or kidney function.

What to Expect Before the Test

No special preparation is usually needed. You may be asked to provide a 24-hour urine sample, so keep track of all urine output over the course of a day. Drink plenty of water to help dilute your urine if instructed.

What the Test Results Mean

Results are typically reported as the concentration of nicotinamide in your urine. Higher than expected levels might suggest certain metabolic disorders or kidney issues, while very low levels could indicate a vitamin B3 deficiency.

Common Conditions Monitored

This test can help monitor conditions like Hartnup disease, which affects how your body absorbs certain nutrients, or other metabolic disorders that may lead to altered vitamin B3 metabolism.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Nicotinamide is a key component in the body’s energy production and DNA repair. Abnormal levels may signal metabolic imbalances, kidney problems, or deficiencies in vitamin B3 intake. Always discuss your results with your healthcare provider to determine the next steps.

Short name

Nicotinamide Urine Test

Frequently asked questions

What does a high nicotinamide level in urine mean?
A high level may indicate conditions like kidney disease or certain metabolic disorders that affect how your body processes vitamin B3. Your provider will consider your overall health and symptoms to interpret results.
What if my results are low?
Low levels might suggest a vitamin B3 deficiency or issues with absorption. Your provider may recommend dietary changes, supplements, or further testing to address the underlying cause.
Can diet affect this test?
Yes, your diet can influence results. High intake of vitamin B3-rich foods or supplements may temporarily increase urine levels. Avoid large changes in your diet before testing, as this could affect accuracy.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy